Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982

See all formats and pricing
In This Section
Volume 43, Issue 10 (Oct 2005)


Anti-inflammatory compound resveratrol suppresses homocysteine formation in stimulated human peripheral blood mononuclear cells in vitro

Katharina Schroecksnadel
  • Division of Biological Chemistry, Biocentre, Innsbruck Medical University, and Ludwig Boltzmann Institute of AIDS-Research, Innsbruck, Austria
/ Christiana Winkler
  • Division of Biological Chemistry, Biocentre, Innsbruck Medical University, and Ludwig Boltzmann Institute of AIDS-Research, Innsbruck, Austria
/ Barbara Wirleitner
  • Division of Biological Chemistry, Biocentre, Innsbruck Medical University, and Ludwig Boltzmann Institute of AIDS-Research, Innsbruck, Austria
/ Harald Schennach
  • Central Institute of Blood Transfusion and Immunology, University Hospital, Innsbruck, Austria
/ Günter Weiss
  • Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria
/ Dietmar Fuchs
  • Division of Biological Chemistry, Biocentre, Innsbruck Medical University, and Ludwig Boltzmann Institute of AIDS-Research, Innsbruck, Austria


Inflammation, immune activation and oxidative stress play a major role in the pathogenesis of cardiovascular disorders. In addition to markers of inflammation, moderate hyperhomocysteinemia is an independent risk factor for cardiovascular disease, and there is a link between the activation of immunocompetent cells and the enhanced formation of homocysteine in vitro. Likewise, anti-inflammatory drugs and nutrients rich in antioxidant vitamins are able to reduce cardiovascular risk and to slow down the atherogenic process. Resveratrol, a phenolic antioxidant synthesized in grapes and vegetables and present in wine, has also been supposed to be beneficial for the prevention of cardiovascular events. Apart from its strong antioxidant properties, resveratrol has also been demonstrated to act as an anti-inflammatory agent. In this study the influence of resveratrol on the production of homocysteine by stimulated human peripheral blood mononuclear cells (PBMCs) was investigated. Results were compared to earlier described effects of the anti-inflammatory compounds aspirin and salicylic acid and of the lipid-lowering drug atorvastatin. Stimulation of PBMCs with the mitogens concanavalin A and phytohemagglutinin induced significantly higher homocysteine accumulation in supernatants compared with unstimulated cells. Treatment with 10–100 μM resveratrol suppressed homocysteine formation in a dose-dependent manner. Resveratrol did not influence the release of homocysteine from resting PBMCs. The data suggest that resveratrol may prevent homocysteine accumulation in the blood by suppressing immune activation cascades and the proliferation of mitogen-driven T-cells. The effect of resveratrol to down-regulate the release of homo-cysteine was comparable to the decline of neopterin concentrations in the same experiments. The suppressive effect of resveratrol was very similar to results obtained earlier with aspirin, salicylic acid and atorvastatin; however, it appeared that doses of compounds needed to reduce homocysteine levels to 50% of stimulated cells were always slightly lower than those necessary to achieve the same effect on neopterin concentrations. The influence of resveratrol and of all the other compounds on homocysteine production appears to be independent of any direct effect on homocysteine biochemistry.

Keywords: homocysteine; immune activation; peripheral blood mononuclear cells (PBMC); resveratrol


  • 1.

  • 2.

  • 3.

  • 4.

  • 5.

  • 6.

  • 7.

  • 8.

  • 9.

  • 10.

  • 11.

  • 12.

  • 13.

  • 14.

  • 15.

  • 16.

  • 17.

  • 18.

  • 19.

  • 20.

  • 21.

  • 22.

  • 23.

  • 24.

  • 25.

  • 26.

  • 27.

  • 28.

  • 29.

  • 30.

  • 31.

  • 32.

  • 33.

  • 34.

  • 35.

About the article

Corresponding author: Dietmar Fuchs, Division of Biological Chemistry, Biocentre, Innsbruck Medical University, Fritz Pregl Str. 3, 6020 Innsbruck, Austria Phone: +43-512-507-3519, Fax: +43-512-507-2865,

Published in Print: 2005-10-01

Citation Information: Clinical Chemical Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.189. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Margarita Rodriguez Brizi, Carla Marrassini, Gabriela Zettler, Graciela Ferraro, and Claudia Anesini
Chinese Medicine, 2012, Volume 03, Number 01, Page 20
Salvatore Chirumbolo
Journal of the Science of Food and Agriculture, 2012, Volume 92, Number 8, Page 1573
Ji Eun Lee, Eunkyo Park, Jung eun Lee, Joong Hyuck Auh, Hyung-Kyoon Choi, Jaehwi Lee, SooMuk Cho, and Jung-Hyun Kim
Nutrition Research and Practice, 2011, Volume 5, Number 5, Page 429
Louis M. Chu, Antonio D. Lassaletta, Michael P. Robich, and Frank W. Sellke
Current Atherosclerosis Reports, 2011, Volume 13, Number 6, Page 439
Christophe Noll, Julien Hamelet, Véronique Ducros, Nicole Belin, Jean-Louis Paul, Jean-Maurice Delabar, and Nathalie Janel
Food and Chemical Toxicology, 2009, Volume 47, Number 1, Page 230
Joanna Malinowska and Beata Olas
Platelets, 2011, Volume 22, Number 4, Page 277
Myung Hee Hong, Jeong-Hyun Kim, Sea Yun Lee, Ho Yeon Go, Ji Hye Kim, Yong-Cheol Shin, Sung-Hoon Kim, and Seong-Gyu Ko
Phytotherapy Research, 2009, Page n/a
Rong Lin, Juntian Liu, Weijie Gan, and Cunjing Ding
Basic & Clinical Pharmacology & Toxicology, 2007, Volume 101, Number 3, Page 197

Comments (0)

Please log in or register to comment.
Log in