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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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Volume 43, Issue 11


Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice

Laurent Ramont
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Henri Thoannes
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ariel Volondat
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ François Chastang
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marie-Christine Millet
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ François-Xavier Maquart
  • Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Reims, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2005-10-19 | DOI: https://doi.org/10.1515/CCLM.2005.210


The concentration of neuron-specific enolase (NSE) in serum and cerebrospinal fluid (CSF) has been used as a biomarker in some cancers and, more recently, in neurodegenerative diseases. Pre-analytical conditions are very important for the quality of returned results. In this study, we evaluated the effects of storage conditions (temperature and duration of storage) and hemolysis on the concentration of NSE in serum and CSF. Our results demonstrate that samples for NSE measurement may be stored at −80°C for no more than 6 months in the case of CSF and 9months in the case of serum samples. Even invisible hemolysis may increase NSE levels in samples. Consequently, an index of hemolysis should be determined before deciding whether or not to perform NSE measurement.

Keywords: cerebrospinal fluid; hemolysis; neuron-specific enolase; serum; storage conditions


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About the article

Corresponding author: Dr. Laurent Ramont, MD, PhD, Laboratoire Central de Biochimie, Hôpital Robert Debré, Centre Hospitalier Universitaire, Avenue du Général Koenig, 51092 Reims Cedex, France Phone: +33-3-26783181, Fax: +33-3-26788539,

Received: 2005-05-24

Accepted: 2005-08-03

Published Online: 2005-10-19

Published in Print: 2005-11-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 43, Issue 11, Pages 1215–1217, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.210.

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