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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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1437-4331
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Volume 43, Issue 2 (Apr 2005)

Issues

Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample

Giuseppe Calcagno
  • Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II” and CEINGE Biotecnologie Avanzate, Naples, Italy
/ Giuseppe Labruna
  • Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II” and CEINGE Biotecnologie Avanzate, Naples, Italy
/ Lucia Sacchetti
  • Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II” and CEINGE Biotecnologie Avanzate, Naples, Italy
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2005.027

Abstract

Given the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex reactions: multiplex A for loci DYS19, DYS385, DYS392 and DYS393, and multiplex B for DYS389 I and II, DYS390, DYS391 and DYS385. The proportion of unique haplotypes was 100% (108 Y-chromosome haplotypes in 108 unrelated males) and the haplotype diversity discrimination index was 0.99. These data reflect the high heterogeneity of male lineage in our population and are similar to those obtained in other regions of southern Italy.

Keywords: capillary electrophoresis; haplotype; short tandem repeats; Y chromosome

References

  • 1

    Beleza S, Alves C, Gonzales-Neira A, Lareu M, Amorim A, Carracedo A, et al. Extending STR markers in Y chromosome haplotypes. Int J Legal Med 2003; 117: 27–33.

  • 2

    Uchihi R, Yamamoto T, Usuda K, Yoshimoto T, Tanaka M, Tokunaga S, et al. Haplotype analysis with 14 Y-STR loci using 2 multiplex amplification and typing system in 2 regional populations in Japan. Int J Legal Med 2003; 117: 34–8.

  • 3

    Jobling MA, Pandya A, Tyler-Smith C. The Y chromosome in forensic analysis and paternity testing. Int J Legal Med 1997; 110: 118–24.

  • 4

    Roewer L, Krawczak M, Willuweit S, Nagy M, Alves C, Amorim A, et al. Online reference database of European Y-chromosomal short tandem repeat (STR) haplotypes. Forensic Sci Int 2001; 118: 106–13.

  • 5

    Parson W, Niederstatter H, Kochl S, Steinlechner M, Berger B. When autosomal short tandem repeats fail: optimized primer and reaction design for Y chromosome short tandem repeat analysis in forensic casework. Croat Med J 2001; 42: 285–7.

  • 6

    Bärr W, Brinkmann B, Budowle B, Gill P, Lincoln P, Mayr W, et al. DNA recommendations. Further report of the DNA commission of the ISFH regarding (STR) haplotypes in European males, use of short tandem repeat systems. Int J Legal Med 1997; 110: 175–6.

  • 7

    Gill P, Brenner C, Brinkmann B, Budowle B, Carracedo A, Jobling MA, et al. DNA commission of the ISFG: recommendations on forensic analysis using Y-chromosome STRs. Int J Legal Med 2001; 114: 305–9.

  • 8

    Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 1973; 70: 3321–3.

  • 9

    Sensabaugh GF. Biochemical markers of individuality. In: Saferstein R, editor. Forensic science handbook. Englewood Cliffs, NJ: Prentice-Hall, 1982:340–415.

  • 10

    Chakravarti A. The effect of linkage on paternity calculations. In: Walker RH, editor. Inclusion probabilities in parentage testing. Arlington, VA: American Association of Blood Banks, 1983:411–20.

  • 11

    Kimura M, Ohta T. Stepwise mutation model and distri-bution of allelic frequencies in a finite population. Proc Natl Acad Sci USA 1978; 75: 2868–72.

  • 12

    Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, et al. Evaluation of Y-chromosomal STRs: a multicenter study. Int J Legal Med 1997; 110: 125–33.

  • 13

    Presciuttini S, Caglià A, Alù M, Asmundo A, Buscemi L, Caenazzo L, et al. Y-Chromosome haplotypes in Italy: the GEFI collaborative database. Forensic Sci Int 2001; 122: 184–8.

About the article

Corresponding author: Lucia Sacchetti, Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II” and CEINGE Biotecnologie Avanzate, via S. Pansini 5, Naples, Italy Fax: +39-081-7462404,


Received: 2004-04-04

Accepted: 2004-11-16

Published Online: 2011-09-21

Published in Print: 2005-04-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.027. Export Citation

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