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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

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Volume 43, Issue 2 (Apr 2005)


Interferences in coagulation tests – evaluation of the 570-nm method on the Dade Behring BCS analyser

Ralf Junker
  • Institute of Arteriosclerosis Research, University of Münster, Münster, Germany and Institute of Clinical Chemistry and Laboratory Medicine, University of Münster, Münster, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Margit Käse / Helmut Schulte / Ruth Bäumer / Claus Langer / Ulrike Nowak-Göttl
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2005.041


The Dade Behring BCS is a coagulation analyser with optical reaction detection (standard 405nm). The present study was conducted to evaluate measurement at 570nm for analyses in interfering plasma samples. Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen and D-dimer in normal (n=50), lipaemic (n=60), icteric (n=113), and haemolytic (n=58) samples were measured at 405 and 570nm. As they are unaffected by the optical properties of the sample, the mechanical STAcompact analyser (Roche Diagnostics) and an ELISA technique were defined as the “comparison” methods. The percentage of valid PT results using the 570-nm method varied from 54% (lipaemic samples) to 97% (haemolytic samples). Valid aPTT measurements were found in 67% (lipaemic samples) up to 93% (icteric samples) of samples. Fibrinogen measurement revealed valid results in 58% (lipaemic samples) to 100% (haemolytic samples) of samples. The number of valid D-dimer results varied from 28% (lipaemic material) up to 100% (haemolytic material). Significant inter-method differences were found: aPTT in lipaemic (BCS 405 vs. 570nm) and icteric samples (STAcompact vs. BCS 405 and 570nm); fibrinogen in lipaemic (BCS 405 vs. 570nm), icteric (BCS 405 vs. 570nm; STAcompact vs. BCS 570nm) and haemolytic samples (STAcompact vs. BCS 405 and 570nm). Differences between the BCS 570-nm and the STAcompact methods were in most cases low and less pronounced than between the BCS 570- and 405-nm methods, making the BCS 570-nm method an alternative to measurement at 405nm. Limitations have to be taken into account regarding lipaemic plasma.

Keywords: coagulation analyser; interference; method evaluation; optical detection


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About the article

Both authors contributed equally to this work; Corresponding author: Ralf Junker, MD, Abendrothsweg 31, 20251 Hamburg, Germany Phone: +49-40-46090175, Fax: +49-40-46093045,

Received: 2004-07-02

Accepted: 2004-12-14

Published Online: 2011-09-21

Published in Print: 2005-04-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.041.

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©2005 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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