Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated technique

  • László Selmeci , Leila Seres , Magda Antal , Júlia Lukács , Andrea Regöly-Mérei and György Acsády

Abstract

Oxidative stress is known to be involved in many human pathological processes. Although there are numerous methods available for the assessment of oxidative stress, most of them are still not easily applicable in a routine clinical laboratory due to the complex methodology and/or lack of automation. In research into human oxidative stress, the simplification and automation of techniques represent a key issue from a laboratory point of view at present. In 1996 a novel oxidative stress biomarker, referred to as advanced oxidation protein products (AOPP), was detected in the plasma of chronic uremic patients. Here we describe in detail an automated version of the originally published microplate-based technique that we adapted for a Cobas Mira Plus clinical chemistry analyzer. AOPP reference values were measured in plasma samples from 266 apparently healthy volunteers (university students; 81 male and 185 female subjects) with a mean age of 21.3years (range 18–33). Over a period of 18months we determined AOPP concentrations in more than 300 patients in our department. Our experiences appear to demonstrate that this technique is especially suitable for monitoring oxidative stress in critically ill patients (sepsis, reperfusion injury, heart failure) even at daily intervals, since AOPP exhibited rapid responses in both directions. We believe that the well-established relationship between AOPP response and induced damage makes this simple, fast and inexpensive automated technique applicable in daily routine laboratory practice for assessing and monitoring oxidative stress in critically ill or other patients.


Corresponding author: Prof. László Selmeci, MD, PhD, Department of Cardiovascular Surgery, Semmelweis University, Faculty of Medicine, Városmajor u. 68, 1122 Budapest, Hungary Fax: +36-1-458-6746,

References

1 Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41: 1819–28. 10.1093/clinchem/41.12.1819Search in Google Scholar

2 Gutteridge JM, Mitchell J. Redox imbalance in the critically ill. Br Med Bull 1999; 55: 49–75. 10.1258/0007142991902295Search in Google Scholar

3 Goodyear-Bruch C, Pierce JD. Oxidative stress in critically ill patients. Am J Crit Care 2002; 11: 543–51. 10.4037/ajcc2002.11.6.543Search in Google Scholar

4 DE Zwart LL, Meerman JH, Commandeur JN, Vermeulen NP. Biomarkers of free radical damage. Applications in experimental animals and in humans. Free Radic Biol Med 1999; 26: 202–26. 10.1016/S0891-5849(98)00196-8Search in Google Scholar

5 Del Rio D, Serafini M, Pellegrini N. Selected methodologies to assess oxidative/antioxidant status in vivo: a criti-cal review. Nutr Metab Cardiovasc Dis 2002; 12: 343–51. Search in Google Scholar

6 Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int 1996; 49: 1304–13. 10.1038/ki.1996.186Search in Google Scholar

7 Witko V, Nguyen AT, Descamps-Latscha B. Microtiter plate assay for phagocyte-derived taurine-chloramines. J Clin Lab Anal 1992; 6: 47–53. 10.1002/jcla.1860060110Search in Google Scholar

8 Capeillère-Blandin C, Gausson V, Descamps-Latscha B, Witko-Sarsat V. Biochemical and spectrophotometric significance of advanced oxidized protein products. Biochim Biophys Acta 2004; 1689: 91–102. 10.1016/j.bbadis.2004.02.008Search in Google Scholar

9 Kaneda H, Taguchi J, Ogasawara K, Aizawa T, Ohno M. Increased level of advanced oxidation protein products in patients with coronary artery disease. Atherosclerosis 2002; 162: 221–5. 10.1016/S0021-9150(01)00706-7Search in Google Scholar

10 Buonocore G, Perrone S, Longini M, Terzuoli L, Bracci R. Total hydroperoxide and advanced oxidation protein products in preterm hypoxic babies. Pediatr Res 2000; 47: 221–4. 10.1203/00006450-200002000-00012Search in Google Scholar PubMed

11 Buonocore G, Perrone S, Longini M, Vezzosi P, Marzocchi B, Paffetti P, et al. Oxidative stress in preterm neonates at birth and on the seventh day of life. Pediatr Res 2002; 52: 46–9. 10.1203/00006450-200207000-00010Search in Google Scholar PubMed

12 Kalousova M, Skrha J, Zima T. Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. Physiol Res 2002; 51: 597–604. Search in Google Scholar

13 Martin-Gallan P, Carrascosa A, Gussinye M, Dominguez C. Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complications. Free Radic Biol Med 2003; 34: 1563–74. 10.1016/S0891-5849(03)00185-0Search in Google Scholar

14 Allanore Y, Borderie D, Lemarechal H, Ekindjian OG, Kahan A. Acute and sustained effects of dihydropyridine-type calcium channel antagonists on oxidative stress in systemic sclerosis. Am J Med 2004; 116: 595–600. 10.1016/j.amjmed.2003.11.022Search in Google Scholar PubMed

15 Sebekova K, Gazdikova K, Syrova D, Blazicek P, Schinzel R, Heidland A, et al. Effects of ramipril in nondiabetic nephropathy: improved parameters of oxidative stress and potential modulation of advanced glycation end products. J Hum Hypertens 2003; 17: 265–70. 10.1038/sj.jhh.1001541Search in Google Scholar PubMed

16 Matteucci E, Biasci E, Giampietro O. Advanced oxidation protein products in plasma: stability during storage and correlation with other clinical characteristics. Acta Diabetol 2001; 38: 187–9. 10.1007/s592-001-8077-3Search in Google Scholar PubMed

Received: 2004-10-12
Accepted: 2004-12-15
Published Online: 2005-6-1
Published in Print: 2005-3-1

©2005 by Walter de Gruyter Berlin New York

Downloaded on 29.3.2024 from https://www.degruyter.com/document/doi/10.1515/CCLM.2005.050/html
Scroll to top button