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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 43, Issue 3

Issues

Evaluation of a high-sensitivity turbidimetric immunoassay for serum C-reactive protein: application to the study of longitudinal changes throughout normal pregnancy

Núria Bertran
  • Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
  • Other articles by this author:
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/ Jordi Camps
  • Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Joan Fernández-Ballart
  • Unitat de Medicina Preventiva i Salut Pública, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Michelle M. Murphy
  • Unitat de Medicina Preventiva i Salut Pública, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Victoria Arija
  • Unitat de Medicina Preventiva i Salut Pública, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Natàlia Ferré
  • Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Mònica Tous
  • Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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/ Jorge Joven
  • Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Universitat Rovira i Virgili, Institut de Recerca en Ciències de la Salut, Reus, Catalunya, Spain
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Published Online: 2014-06-05 | DOI: https://doi.org/10.1515/CCLM.2005.053

Abstract

C-Reactive protein has been associated with several complications of pregnancy. The aims of the present study were: (1) to evaluate a turbidimetric immunoassay for the measurement of C-reactive protein; and (2) to investigate the chronological changes of the levels of this protein from preconception throughout normal pregnancy and its relationship with variables associated with preconception and pregnancy outcome. Inter-assay imprecision was <5% for C-reactive protein >1mg/L and 18% at a mean value of 0.33mg/L. The limit of detection was 0.10mg/L. The method was linear between 0.10 and 30mg/L. There were no observed interferences from jaundice, hemolysis, lipemia or paraproteinemia at the levels studied. There was good agreement with the nephelometric method. A total of 39 women were studied at preconception, at 8, 20 and 32weeks of pregnancy, and in labor. Preconception C-reactive protein concentration was 1.17±0.18mg/L and increased (p<0.001) throughout pregnancy up to 5.69±0.82mg/L. Body mass index at preconception and weight gain during pregnancy were the main factors associated with this increase in C-reactive protein.

Keywords: C-reactive protein; inflammation; method evaluation; pregnancy

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About the article

Corresponding author: Dr. Jordi Camps, Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, C/. Sant Joan s/n, 43201-Reus, Catalunya, Spain Phone: +34-977-310300, Fax: +34-977-312569,


Received: 2004-11-15

Accepted: 2005-01-12

Published Online: 2014-06-05

Published in Print: 2005-03-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 43, Issue 3, Pages 308–313, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.053.

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