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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Volume 43, Issue 6


A simple method for estimating equilibrium constants for serum testosterone binding resulting in an optimal free testosterone index for use in elderly men

H. Alec Ross
  • Department of Chemical Endocrinology and Department of Endocrinology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eric J. Meuleman / Fred C. G. J. Sweep
  • Department of Chemical Endocrinology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-11 | DOI: https://doi.org/10.1515/CCLM.2005.106


An algorithm was developed to evaluate equilibrium constants for testosterone (Te) and sex hormone-binding globulin (SHBG) or albumin from serum free testosterone (FTe) measurements performed in a panel of 30 healthy elderly men by means of a near-reference method, i.e., symmetric dialysis (affinity constants: SHBG-Te, 1.13×10 9L/mol; albumin-Te, 4.4×10 4L/mol). Using these estimates, a free testosterone index (FTeI) was calculated from total Te and SHBG concentrations in a further 35 elderly men. This FTeI perfectly matches with actually measured free testosterone concentrations by symmetric dialysis in this second group, with a mean ratio index/measurement of 0.998±0.016 (SEM). The efficacy of the algorithm, which represents a simple alternative to previous cumbersome methods for estimation of equilibrium constants, is thereby demonstrated.

Keywords: dialysis; equilibrium constant; free testosterone; sex hormone-binding globulin.


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About the article

Corresponding author: H. Alec Ross, 530 Department of Chemical Endocrinology, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands Fax: +31-24-3541484,

Received: 2004-12-08

Accepted: 2005-03-30

Published Online: 2014-06-11

Published in Print: 2005-06-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 43, Issue 6, Pages 613–616, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2005.106.

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