Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 44, Issue 10 (Oct 2006)

Issues

Multiplexed testing in the autoimmunity laboratory

José M. González-Buitrago
  • Laboratorio de Autoinmunidad, Servicio de Bioquímica Clínica, Hospital Universitario de Salamanca, y Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2006-10-10 | DOI: https://doi.org/10.1515/CCLM.2006.213

Abstract

A variety of technologies for autoantibody profiling have been developed. The main techniques are line-blot immunoassays, bar-coded nanoparticle immunoassays, and bead-based assays with flow cytometry detection and antigen microarrays. Some of these technologies are only able to measure a limited number of autoantibodies, while others can detect elevated numbers. Assays for antinuclear antibody specificities using line-blot immunoassays and bead-based assays with flow cytometry detection are already commercialised. Antigen microarrays for autoantibody measurement are only in the development phase, although in the not too distant future these assays will probably appear on the market. Multiplexed testing in the autoimmunity laboratory appears to have a promising future, since this technique permits a reduction in analytical time, with a shorter turnaround time.

Clin Chem Lab Med 2006;44:1169–74.

Keywords: antigen microarray; antinuclear antibodies; autoantibody; autoimmune diseases; multiplexed tests

References

  • 1.

    Davidson A, Diamond B. Autoimmune diseases. N Engl J Med 2001; 345:340–50.CrossrefGoogle Scholar

  • 2.

    Marrack P, Kappler J, Kotzin BL. Autoimmune disease: why and where it occurs. Nature Med 2001; 7:899–905.CrossrefGoogle Scholar

  • 3.

    Hochberg M. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1997; 40:1725.CrossrefGoogle Scholar

  • 4.

    Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander E, Carsons SE, et al. Classification criteria for Sjögren's syndrome: a revised version of the European criteria proposed by the American-European consensus group. Ann Rheum Dis 2002; 61:554–8.CrossrefGoogle Scholar

  • 5.

    Sherer Y, Gorstein A, Fritzler MJ, Shoenfeld Y. Autoantibody explosion in systemic lupus erythematosus: more than 100 different antibodies found in SLE patients. Semin Arthritis Rheum 2004; 34:501–37.CrossrefGoogle Scholar

  • 6.

    Arbuckle MR, James JA, Dennis GJ, Rubertone HV, McClain MT, Kim XR, et al. Development of autoantibodies before the clinical onset of systemic lupus erythematosus. N Engl J Med 2003; 349:1526–33.Google Scholar

  • 7.

    Verge CF, Gianani R, Kawasaki E, Yu L, Pietropaolo M, Chase HP, et al. Number of autoantibodies (against insulin, GAD or ICA512/IA2) rather than particular autoantibody specificities determines risk of type I diabetes. J Autoimmun 1996; 9:379–83.Google Scholar

  • 8.

    González-Buitrago JM, González C. Present and future of the autoimmunity laboratory. Clin Chim Acta 2006; 365:50–7.Google Scholar

  • 9.

    Meheus L, van Venrooij WJ, Wiik A, Charles PJ, Tzioufas AG, Meyer O, et al. Multicenter validation of recombinant, natural and synthetic antigens in a single multiparameter assay for the detection of specific antinuclear autoantibodies in connective tissue disorders. Clin Exp Rheumatol 1999; 17:205–14.Google Scholar

  • 10.

    Pottel H, Wiik A, Locht H, Gordon T, Roberts-Thomson P, Abraham O, et al. Clinical optimization and multicenter validation of antigen-specific cut-off values on the INNO-LIA ANA update for the detection of autoantibodies to connective tissue disorders. Clin Exp Rheumatol 2004; 22:579–88.Google Scholar

  • 11.

    Damoiseaux J, Boesten K, Giesen J, Austen J, Cohen Tervaert JW. Evaluation of a novel line-blot immunoassay for the detection of antibodies to extractable nuclear antigens. Ann NY Acad Sci 2005; 1050:340–7.Google Scholar

  • 12.

    Eissfeller P, Sticherling M, Scholz D, Hennig K, Lüttich T, Motz M, et al. Comparison of different test system for simultaneous autoantibody detection in connective tissue diseases. Ann NY Acad Sci 2005; 1050:327–39.Google Scholar

  • 13.

    Penn SG, He L, Natan MJ. Nanoparticles for bioanalysis. Curr Opin Chem Biol 2003; 7:609–15.CrossrefGoogle Scholar

  • 14.

    Smith J, Onley D, Garey C, Crowther S, Cahir N, Johanson A, et al. Determination of ANA specificity using the UltraPlex™ Platform. Ann NY Acad Sci 2005; 1050:286–94.Google Scholar

  • 15.

    Rouquette AM, Desgruelles C, Laroche P. Evaluation of the new multiplexed immunoassay, FIDIS, for simultaneous quantitative determination of antinuclear antibodies and comparison with conventional methods. Am J Clin Pathol 2003; 120:676–81.CrossrefGoogle Scholar

  • 16.

    Buliard A, Fortenfant F, Ghillani-Dalbin P, Musset L, Oksman F, Olsson NO. [Analysis of nine autoantibodies associated with systemic autoimmune diseases using the Luminex technology. Results of a multicenter study]. Ann Biol Clin (Paris) 2005; 63:51–8.Google Scholar

  • 17.

    Martins TB, Burlingame R, von Muhlen CA, Jaskowski TD, Litwin CM, Hill HR. Evaluation of multiplexed fluorescent microsphere immunoassay for detection of autoantibodies to nuclear antigens. Clin Diagn Lab Immunol 2004; 11:1054–9.Google Scholar

  • 18.

    Gilburd B, Abu-Shakra M, Shoenfeld Y, Giordano A, Bocci EB, delle Monache F, et al. Autoantibodies profile in the sera of patients with Sjögren's syndrome: the ANA evaluation – a homogeneous, multiplexed system. Clin Dev Immunol 2004; 11:53–6.CrossrefGoogle Scholar

  • 19.

    Shovman O, Gilburd B, Zandman-Goddard G, Yehiely A, Langevitz P, Shoenfeld Y. Multiplexed Athena Multi-Lyte immunoassay for ANA screening in autoimmune diseases. Autoimmunity 2005; 38:105–9.CrossrefGoogle Scholar

  • 20.

    Zandman-Goddard G, Gilburd B, Showman O, Blank M, Berdichevski S, Langevitz P, et al. The homogeneous multiplexed system – a new method for autoantibody profile in systemic lupus erythematosus. Clin Dev Immunol 2005; 12:107–11.CrossrefGoogle Scholar

  • 21.

    Nifli AP, Notas G, Mamoulaki M, Niniraki M, Ampartzaki V, Theodoropoulos PA, et al. Comparison of a multiplex, bead-based fluorescent assay and immunofluorescence methods for the detection of ANA and ANCA autoantibodies in human serum. J Immunol Methods 2006; 311:189–97.Google Scholar

  • 22.

    Shovman O, Gilburd B, Barzilai O, Shinar E, Larida B, Zandman-Goddard G, et al. Evaluation of the BioPlex™ 2200 ANA screen. Analysis of 510 healthy subjects: incidence of natural/predictive autoantibodies. Ann NY Acad Sci 2005; 1050:380–8.Google Scholar

  • 23.

    González C, García-Berrocal B, Talaván T, Casas ML, Navajo JA, González-Buitrago JM. Clinical evaluation of a microsphere bead-based flow cytometry assay for the simultaneous determination of anti-thyroid peroxidase and anti-thyroglobulin antibodies. Clin Biochem 2005; 38:966–72.CrossrefGoogle Scholar

  • 24.

    Balboni I, Chan SM, Kattah M, Tenenbaum JD, Butte AJ, Utz PJ. Multiplexed protein array platforms for analysis of autoimmune diseases. Annu Rev Immunol 2006; 24:391–418.CrossrefWeb of ScienceGoogle Scholar

  • 25.

    Joos TO, Schrenk M, Höpfl P, Kroger K, Chowdhury U, Stoll D, et al. A microarray enzyme-linked immunosorbent assay for autoimmune diagnostics. Electrophoresis 2000; 21:2641–50.CrossrefGoogle Scholar

  • 26.

    Robinson WH, DiGennaro C, Hueber W, Haab BB, Kamachi M, Dean EJ, et al. Autoantigen microarrays for multiplex characterisation of autoantibody responses. Nat Med 2002; 8:295–301.CrossrefGoogle Scholar

  • 27.

    Hueber W, Kidd BA, Tomooka BH, Lee BJ, Bruce B, Fries JF. Antigen microarray profiling of autoantibodies in rheumatoid arthritis. Arthritis Rheum 2005; 52:2645–55.CrossrefGoogle Scholar

  • 28.

    Feng Y, Ke X, Ma R, Chen Y, Hu G, Lu F. Parallel detection of autoantibodies with microarrays in rheumatoid diseases. Clin Chem 2004; 50:416–22.CrossrefGoogle Scholar

  • 29.

    Robinson WH, Steinman L, Utz PJ. Proteomics technologies for the study of autoimmune disease. Arthritis Rheum 2002; 46:855–93.CrossrefGoogle Scholar

  • 30.

    Hueber W, Utz PJ, Steinman L, Robinson WH. Autoantibody profiling for the study and treatment of autoimmune disease. Arthritis Res 2002; 4:290–5.CrossrefGoogle Scholar

  • 31.

    Robinson WH, Steinman L, Utz PJ. Protein and peptide array analysis of autoimmune disease. Biotechniques 2002; 33:S66–9.Google Scholar

  • 32.

    Robinson WH, Utz PJ, Steinman L. Genomic and proteomic analysis of multiple sclerosis. Opinion. Curr Opin Immunol 2003; 15:660–7.CrossrefGoogle Scholar

  • 33.

    Robinson WH, Steinman L, Utz PJ. Protein arrays for autoantibody profiling and fine-specificity mapping. Proteomics 2003; 3:2077–84.CrossrefGoogle Scholar

  • 34.

    Graham KL, Robinson WH, Steinman L, Utz PJ. High-throughput methods for measuring autoantibodies in systemic lupus erythematosus and other autoimmune diseases. Autoimmunity 2004; 37:269–72.CrossrefGoogle Scholar

  • 35.

    Robinson WH. Antigen arrays for autoantibody profiling. Curr Opin Chem Biol 2006; 10:67–72.CrossrefGoogle Scholar

About the article

Correspondence to: Prof. José M. González-Buitrago, Servicio de Bioquímica, Hospital Universitario de Salamanca, Salamanca, Spain Fax: +34-923-291211,


Received: 2006-05-31

Accepted: 2006-06-21

Published Online: 2006-10-10

Published in Print: 2006-10-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.213.

Export Citation

©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Robin Jarquin, Irene Hanning, Soohyoun Ahn, and Steven C. Ricke
Sensors, 2009, Volume 9, Number 7, Page 5308
[2]
Si Hong Park, Muhsin Aydin, Anita Khatiwara, Maureen C. Dolan, David F. Gilmore, Jennifer L. Bouldin, Soohyoun Ahn, and Steven C. Ricke
Food Microbiology, 2014, Volume 38, Page 250
[3]
Ángel San Miguel Hernández, Rafael San Miguel, and Francisco Javier Martín-Gil
Revista del Laboratorio Clínico, 2010, Volume 3, Number 1, Page 40
[4]
Ángel San Miguel-Hernández, Francisco J. Martín-Gil, and Alicia Armentia-Medina
Diálisis y Trasplante, 2009, Volume 30, Number 4, Page 139
[5]
Luca Giovanella, Emanuela Toffalori, Renato Tozzoli, Marco Caputo, Luca Ceriani, and Frederik A. Verburg
Head & Neck, 2012, Volume 34, Number 10, Page 1369
[6]
Mario Plebani, Marina Pittoni, Marilena Celadin, Daniela Bernardi, and Monica Maria Mion
Autoimmunity Reviews, 2009, Volume 8, Number 3, Page 238
[7]
A. Tedesco, D. D'Agostino, I. Soriente, P. Amato, R. Piccoli, and P. Sabatini
Autoimmunity Reviews, 2009, Volume 8, Number 3, Page 233
[8]
Renato Tozzoli
Clinical Chemistry and Laboratory Medicine, 2008, Volume 46, Number 5
[10]
Markus Thaler, Alexander Buhl, Harald Welter, Anita Schreiegg, Marcus Kehrel, Bettina Alber, Jochen Metzger, and Peter B. Luppa
Analytical and Bioanalytical Chemistry, 2009, Volume 393, Number 5, Page 1417

Comments (0)

Please log in or register to comment.
Log in