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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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1437-4331
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Volume 44, Issue 11 (Nov 2006)

Issues

Automation and validation of a fast method for the assessment of in vivo oxidative stress levels

Cristina Vassalle
  • CNR Institute of Clinical Physiology, Pisa, Italy
/ Claudio Boni
  • CNR Institute of Clinical Physiology, Pisa, Italy
/ Pietro Di Cecco
  • CNR Institute of Clinical Physiology, Pisa, Italy
/ Rudina Ndreu
  • CNR Institute of Clinical Physiology, Pisa, Italy
/ Gian Carlo Zucchelli
  • CNR Institute of Clinical Physiology, Pisa, Italy
Published Online: 2006-11-07 | DOI: https://doi.org/10.1515/CCLM.2006.243

Abstract

Background: The d-ROMs test for the evaluation of serum hydroperoxides (HP) is simple, reliable, and cheap. Furthermore, it can easily be adapted to automated analyzers. Changing from the manual to an automated procedure allows the simultaneous processing of a large number of samples in a greatly reduced time, avoiding manual handling of samples and reagents and reducing variability sources.

Methods: This study was performed to adjust the manual procedure to a routine automated method in the clinical laboratory. We carried out the d-ROMs test in sera from 90 subjects of both sexes (34 men and 56 women) with age ranging from 20 to 80years (mean 51±14years). All subjects were free from acute or chronic inflammatory disease, immunological disease and history or evidence of malignancy. Subjects were not on vitamin and/or antioxidant therapies.

Results: The detection limit of the assay was 40AU. Linearity was observed up to 475AU. The recovery ranged between 97% and 105%. Within- and between-run imprecision was <5%. The mean HP value was 304±8AU, with no significant difference between men (291±10AU) and women (311±11AU). A significant positive correlation was observed between age and HP in the whole population (r=0.4, p=0.0002).

Conclusions: The automated test for the estimation of serum hydroperoxides represents a reliable and feasible procedure for increasing efficiency and reducing costs compared to the manual method, and is particularly suitable for evaluating oxidative stress in a variety of clinical conditions.

Clin Chem Lab Med 2006;44:1372–5.

Keywords: age; automated procedure; gender; manual method; oxidative stress

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About the article

Corresponding author: Dr. Cristina Vassalle, PhD, Istituto di Fisiologia Clinica-Area di ricerca CNR, Via Moruzzi 1, 56124, Pisa, Italy Phone: +39-050-3152199, Fax:+39-050-3152166


Received: 2006-05-26

Accepted: 2006-08-23

Published Online: 2006-11-07

Published in Print: 2006-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.243. Export Citation

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