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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

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1437-4331
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Volume 44, Issue 12

Issues

Variations in assay protocol for the Dako cystatin C method may change patient results by 50% without changing the results for controls

Mats Flodin / Lars-Olof Hansson / Anders Larsson
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.271

Abstract

Background: Cystatin C is increasingly used as a glomerular filtration marker, but so far only a few companies produce most of the cystatin C reagents suited for turbidimetry or nephelometry use in clinical laboratories.

Methods: We studied different protocols for measuring cystatin C on an Architect ci8200 system using cystatin C reagents from Dako (Glostrup, Denmark). The results were compared with those obtained with Dade Behring reagents (Deerfield, IL, USA) on a BN ProSpec system.

Results: Differences in assay protocol on the same instrument with the Dako reagent yielded an up to 50% difference in glomerular filtration rate calculated from the cystatin C results when analyzing patient samples, but had no effect on the results for controls. There were also significant differences regarding linearity and kinetics between samples and controls/calibrators.

Conclusions: The results indicate different reactivity of the Dako antibodies against calibrators and controls in comparison with patient samples, highlighting the importance of using controls and calibrators that do not differ from patient samples.

Clin Chem Lab Med 2006;44:1481–5.

Keywords: cystatin C; erroneous results; glomerular filtration ratio; immunoassay; kidney function; recombinant proteins; turbidimetry

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About the article

Corresponding author: Anders Larsson, Department of Medical Sciences, University Hospital, 751 85 Uppsala, Sweden Phone: +46-18-6110000, Fax: +46-18-552562,


Received: 2006-03-16

Accepted: 2006-09-26

Published Online: 2011-09-21

Published in Print: 2006-12-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 12, Pages 1481–1485, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.271.

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©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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