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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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IMPACT FACTOR 2016: 3.432

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Volume 44, Issue 2 (Feb 2006)


Establishment of an indocyanine green test using an automatic chemistry analyzer

Moon-Woo Seong / Sang-Hoon Song / Joo-Young Oh / Joong-Won Park / Do-Hoon Lee
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.036


The indocyanine green (ICG) clearance test has been used to assess the reserve of hepatic function. This method is based on the spectrometric measurement of its plasma concentration at maximum wavelength of 805nm, which requires a spectrophotometer and associated maintenance. We established an ICG clearance test using a Toshiba 200FR automatic chemistry analyzer that can be tuned to a wavelength of approximately 805nm. Five pooled sera spiked from 0 to 4.0mg/dL were analyzed for linearity test and precision was determined at five levels in the range 0.1–2.0mg/dL. The ICG retention rate at 15min (R15) was determined for 38 patients using a conventional method and our method. The ICG clearance test using the automatic chemistry analyzer showed good linearity, and precision ranged from 0.3% to 1.0% for within-run CVs and from 0.6% to 4.7% for total CVs. The degree of agreement between the two methods was also acceptable (mean difference of 1.5%). It is expected that the ICG test using the automatic chemistry analyzer can replace the conventional ICG clearance test, considering the excellent agreement, good precision and linearity over a clinically relevant range.

Keywords: comparison; hepatic function; indocyanine green (ICG); TBA 200FR spectrophotometer


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About the article

Corresponding author: Dr. Do-Hoon Lee, Center for Clinical Services, National Cancer Center, 809 Madu-1-dong, Ilsandong-gu, Goyang 410-769, Korea Phone: +82-31-920-1734, Fax: +82-31-920-1738,

Received: 2005-09-12

Accepted: 2005-11-15

Published Online: 2011-09-21

Published in Print: 2006-02-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.036.

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©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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