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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 44, Issue 2

Issues

Measurement of glycated hemoglobin (HbA1c) with an automated POCT instrument in comparison with HPLC and automated immunochemistry method: evaluation of the influence of hemoglobin variants

Alexander Haliassos / Ioannis Drakopoulos / Demosthenes Katritsis / Nikolaos Chiotinis / Socrates Korovesis / Konstantinos Makris
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.041

Abstract

Our study evaluates a fast and easy way to perform point-of-care testing (POCT) measurements of glycated hemoglobin HbA1c in comparison with an immunoassay on an automated biochemistry analyzer and cation exchange chromatography, the two methods routinely used in clinical laboratories for the measurement of HbA1c. A significant finding of our study is that although the POCT instrument insert claims that the method is not affected by the presence of HbS in the heterozygous state or in combination with β-thalassemia, discrepant results were found in some cases with such hemoglobinopathies. In these cases, the two POCT and laboratory immunoassay methods showed clinically significant positive interferences with samples containing the HbS trait. We conclude that samples with the HbS trait should be interpreted with caution when tested using the POCT instrument.

Keywords: glycated hemoglobin (HbA1c); method comparison; POCT instruments

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About the article

Corresponding author: Dr. A. Haliassos, Central Laboratories, Athens Euroclinic, 9 Athanassiadou St, Athens 11521, Greece Phone: +30-210-6416380, Fax: +30-210-9674137,


Received: 2005-09-19

Accepted: 2005-11-03

Published Online: 2011-09-21

Published in Print: 2006-02-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 2, Pages 223–227, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.041.

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