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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Volume 44, Issue 3 (Mar 2006)


Time-resolved fluorimetric immunoassay of calprotectin: technical and clinical aspects in diagnosis of inflammatory bowel diseases

Gertjan van der Sluijs Veer
  • Department of Clinical Chemistry, Medisch Spectrum Twente Hospital, Enschede, The Netherlands
/ Barbara van den Hoven
  • Department of Clinical Chemistry, Medisch Spectrum Twente Hospital, Enschede, The Netherlands
/ Maurice G.V.M. Russel
  • Department of Internal Medicine, Medisch Spectrum Twente Hospital, Enschede, The Netherlands
/ Frank A.J.T.M. van den Bergh
  • Department of Clinical Chemistry, Medisch Spectrum Twente Hospital, Enschede, The Netherlands
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.051


Background: Growing evidence in the literature shows the clinical importance of the calprotectin assay in faeces, especially for the differential diagnosis and monitoring of patients with inflammatory bowel diseases.

Methods: We developed a time-resolved fluorimetric immunoassay for calprotectin to extend the limited measuring range of the commercially available ELISA method currently used in our laboratory. Together with the introduction of a non-enzymatic label, this new method offers the advantages of better precision and higher sensitivity.

Results: The new assay shows a dynamic measuring range extended by a factor four, which reduces the number of samples with concentrations outside the measuring range from 30% to only 4%. The value of the assay was confirmed in various patient groups suffering from active and inactive gastrointestinal diseases. We suggest that the frequent coincidence of a high calprotectin concentration with intestinal blood loss is not the consequence of mere blood loss, but can be ascribed to neutrophil infiltration and subsequent shedding into the intestinal lumen as a result of intestinal inflammation or malignancy.

Conclusion: We expect that in the near future faecal calprotectin will be used as a non-invasive routine diagnostic marker and an effective laboratory parameter to monitor patients with inflammatory bowel disease.

Keywords: calprotectin; Crohn's disease; inflammatory bowel disease; irritable bowel syndrome; time-resolved fluorimetric immunoassay; ulcerative colitis


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About the article

Corresponding author: G. van der Sluijs Veer, Medisch Spectrum Twente, Laboratorium, Postbus 50000, 7500 KA, Enschede, The Netherlands Phone: +31-53-4873400, Fax: +31-53-4873075,

Received: 2005-10-06

Accepted: 2005-12-05

Published Online: 2011-09-21

Published in Print: 2006-03-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.051.

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©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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