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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Volume 44, Issue 3 (Mar 2006)


Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing and Working Group on Selective Electrodes

Mohammed C. Ben Rayana
  • Laboratory of Clinical Chemistry, National Institute of Nutrition, Bab Saadoun, Tunis, Tunisia
/ Robert W. Burnett
  • Department of Pathology, Hartford Hospital, Hartford, CT, United States of America
/ Arthur K. Covington
  • Department of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
/ Paul D'Orazio
  • Instrumentation Laboratory, Lexington, MA, United States of America
/ Niels Fogh-Andersen
  • Laboratory of Clinical Chemistry, Herlev Hospital, Herlev, Denmark
/ Ellis Jacobs
  • Clinical Laboratory Program, Wadsworth Center, NY State Department of Health, Albany, NY, United States of America
/ Ritu Kataky
  • Department of Chemistry, University of Durham, Durham, UK
/ Wolf R. Külpmann
  • Klinische Chemie, Medizinische Hochschule, Hannover, Germany
/ Katsuhiko Kuwa
  • Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan
/ Lasse Larsson
  • Department of Clinical Chemistry, University of Linköping, Sweden
/ Andrzej Lewenstam
  • Center ProSens, Åbo Akademi University, Åbo-Turku, Finland
/ Anton H.J. Maas
  • Eurotrol bv, Ede, Netherlands
/ Gerhard Mager
  • Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany
/ Jerzy W. Naskalski
  • Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Krakow, Poland
/ Anthony O. Okorodudu
  • Department of Pathology, John Sealy Hospital, Galveston, TX, United States of America
/ Christoph Ritter
  • Roche Diagnostics GmbH, Graz, Austria
/ Andrew St John
  • ARC Consulting, 14 Learoyd Street, MT Lawley, Australia
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.060


The proposed recommendation for measuring and reporting chloride in undiluted plasma or blood by ion-selective electrodes (ISEs) will provide results that are identical to chloride concentrations measured by coulometry for standardized normal plasma or blood samples. It is applicable to all current ISEs dedicated to chloride measurement in undiluted samples that meet the requirements. However, in samples with reduced water concentration, results by coulometry are lower than by ion-selective electrode due to volume displacement. The quantity measured by this standardized ISE procedure is called the ionized chloride concentration. It may be clinically more relevant than the chloride concentration as determined by coulometry, photometry or by ISE after dilution of the sample.

Keywords: blood; chloride (ionized and total); coulometry; ion-selective electrode; plasma; standardization


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About the article

Corresponding author: Prof. Dr. W.R. Külpmann, Klinische Chemie, Medizinische Hochschule Hannover, Carl-Neuberg-Str, 1, 30625 Hannover, Germany Phone: +49-5115326613, Fax: +49-5115328614,

Published Online: 2011-09-21

Published in Print: 2006-03-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.060.

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©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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