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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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Volume 44, Issue 4


Matrix metalloproteinases and their inhibitors in different acute stroke subtypes

Ines Vukasovic / Andrea Tesija-Kuna / Elizabeta Topic / Visnja Supanc / Vida Demarin / Marija Petrovcic
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.079


The aim of the study was to determine serum levels of selected matrix metalloproteinases (MMPs) and their natural inhibitors (TIMPs) in the acute phase of different stroke types subdivided according to the Oxfordshire Community Stroke Project (OCSP) classification and the possibility of discriminating stroke types according to their levels. The study included 126 patients with acute stroke within the first 24h of symptom onset, and 124 healthy volunteers. The stroke group had lower MMP-2 concentrations and MMP-2/TIMP-2 ratios (p<0.001) but higher TIMP-2 (p<0.001) than controls. The level of MMP-9 and the MMP-9/TIMP-1 ratio were higher in patients with total anterior circulation infarct (TACI) than in patients with other stroke subtypes according to OCSP classification (p=0.0019, p=0.0065, respectively) or in controls (p<0.0001, p=0.0024, respectively). A negative correlation of MMP-2 levels with MMP-9 and MMP-9/TIMP-1 ratio was recorded in all stroke subtypes except for TACI. Receiver operating characteristic analysis showed similar discriminating power for MMP-9 levels and Barthel index in the differential diagnosis of TACI. High MMP-9/TIMP-1 ratio (odds ratio 3.263) was associated with TACI. Our results demonstrate that the MMP-9/TIMP-1 ratio may provide information to help in assessing stroke patients in the future as a baseline biomarker of infarct extent.

Keywords: matrix metalloproteinases; stroke; tissue inhibitor of metalloproteinases


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About the article

Corresponding author: Ines Vukasovic, Clinical Institute of Chemistry, Sestre milosrdnice University Hospital, Vinogradska c. 29, 10000 Zagreb, Croatia Phone: +385-1-3787381, Fax: +385-1-3768280,

Received: 2005-10-19

Accepted: 2006-01-10

Published Online: 2011-09-21

Published in Print: 2006-04-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 4, Pages 428–434, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.079.

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