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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

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Volume 44, Issue 4


Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate: a pilot study

Fabrizia Bamonti
  • Department of Medical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cristina Novembrino
  • Department of Surgical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Silvia Ippolito
  • Department of Medical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Enzo Soresi / Alberto Ciani
  • Department of Medical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Silvia Lonati
  • Department of Medical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elisabetta Scurati-Manzoni
  • Department of Medical Science, University of Milan, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Milan, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Giuliana Cighetti
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2006.084


Background: Cigarette smoking, a cardiovascular risk factor leading to oxygen free radical formation, is involved in the development of serious pathological conditions. On the other hand, a healthy diet and adequate supplementation can help prevent many diseases. The aim of our study was to evaluate in healthy light smokers the effects of supplementation with mixed fruit and vegetable juice powder concentrate on homocysteine metabolism and oxidative status.

Methods: In this pilot study, 32 healthy volunteers, 16 light smokers and 16 non-smokers, on twice daily supplementation were monitored at time zero and after 30days. Plasma homocysteine, and serum vitamin B12 and folate concentrations were measured by immunoenzymatic assays; reactive oxygen species, total antioxidant capacity and thiol groups by spectrophotometric methods; and total and free malondialdehyde concentrations by gas chromatography-mass spectrometry with isotopic dilution.

Results: Baseline free malondialdehyde concentrations were significantly higher in smokers than in non-smokers and normalised after 30-day supplementation. Baseline results for all the other parameters remained unchanged after supplementation, with no significant differences between smokers and non-smokers.

Conclusion: This is the first study showing a significant decrease in free malondialdehyde levels in light smokers after 1-month phytonutrient supplementation.

Keywords: homocysteine; malondialdehyde; oxidative stress; phytonutrients; smoking


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About the article

Corresponding author: Fabrizia Bamonti, Dipartimento Scienze Mediche, Università degli Studi di Milano, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Fondazione IRCCS, Padiglione Granelli, Via F. Sforza, 35, 20122 Milano, Italy Phone: +39-02-55033473, Fax: +39-02-55034736 ,

Received: 2005-07-28

Accepted: 2006-01-18

Published Online: 2011-09-21

Published in Print: 2006-04-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 4, Pages 391–395, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.084.

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