Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 44, Issue 7 (Jul 2006)

Issues

Autoantibody profiling of patients with autoimmune thyroid disease using a new multiplexed immunoassay method

Renato Tozzoli / Danilo Villalta / Graziano Kodermaz / Marcello Bagnasco / Elio Tonutti / Nicola Bizzaro
Published Online: 2006-06-16 | DOI: https://doi.org/10.1515/CCLM.2006.137

Abstract

Background: It has been proposed that only anti-thyroid-peroxidase (TPOAb) and not anti-thyroglobulin (TgAb) antibodies should be assayed in the diagnosis of autoimmune thyroid disease (AITD).

Methods: We analyzed the thyroid autoantibody profile with a multiplex bead array system, which allows simultaneous measurement of thyroid autoantibodies (FIDIS, BioMedical Diagnostics, Marne La Vallée, France). A total of 151 AITD patients (111 Hashimoto's thyroiditis and 40 Graves' disease) and 414 control patients were recruited to the study.

Results: The diagnostic sensitivity of TPOAb was greater than that of TgAb in both Hashimoto's thyroiditis (91.9% vs. 82.5%) and Graves' disease (82.5% and 52.5%, respectively), whereas the diagnostic specificity was similar (92.7% for TPOAb and 92.4% for TgAb).

Conclusions: The presence of isolated positivity for TgAb in 6% of the Hashimoto's thyroiditis patients indicates that the use of only the TPOAb test does not guarantee sufficient diagnostic sensitivity. In the light of recent findings relating to the pathogenetic role of TgAb and TPOAb in Hashimoto's thyroiditis, knowledge of the autoantibody profile could allow the identification of sub-groups of patients with a different degree of activation of the thyroid autoimmune process.

Clin Chem Lab Med 2006;44:837–42.

Keywords: Graves' disease; Hashimoto's thyroiditis; multiplex bead array; thyroglobulin antibodies; thyroperoxidase antibodies

References

  • 1.

    Cooper GS, Strehla BC. The epidemiology of autoimmune diseases. Autoimmun Rev 2003; 2:119–25.CrossrefGoogle Scholar

  • 2.

    Jacobson DL, Gange SJ, Rose NR, Graham NM. Epidemiology and estimated population burden of selected autoimmune diseases in the United States. Clin Immunol Immunopathol 1997; 84:223–43.CrossrefGoogle Scholar

  • 3.

    Baloch Z, Carayon P, Conte-Devolx B, Demers LM, Feldt-Rasmussen U, Henry JF, et al. Laboratory medicine practice guidelines. Laboratory support for the diagnosis and monitoring of thyroid disease. Thyroid 2003; 13:3–126.Google Scholar

  • 4.

    Hollowell J, Staehling NW, Flanders WD, Hannon VH, Gunter EW, Spencer C, et al. Serum TSH, T4, and thyroid antibodies in the United States population (1988 to 1994): National Health and Nutrition Examination Survey (NHANES III). J Clin Endocrinol Metab 2002; 87:489–99.Google Scholar

  • 5.

    Mariotti S, Caturegli P, Piccolo P, Barbesino G, Pinchera A. Antithyroid peroxidase autoantibodies in thyroid diseases. J Clin Endocrinol Metab 1990; 71:661–9.CrossrefGoogle Scholar

  • 6.

    Doullay F, Ruf J, Codaccioni JL, Carayon P. Prevalence of autoantibodies to thyroperoxidase in patients with various thyroid and autoimmune diseases. Autoimmunity 1991; 9:237–44.CrossrefGoogle Scholar

  • 7.

    Feldt-Rasmussen U, Hoier-Madsen M, Bech K, Blichert-Toft M, Bliddal H, Date J, et al. Anti-thyroid peroxidase antibodies in thyroid disorders and non thyroid autoimmune diseases. Autoimmunity 1991; 9:245–51.CrossrefGoogle Scholar

  • 8.

    Czarnocka B, Ruf J, Ferrand J, Carayon P, Lissitzky S. Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases. FEBS Lett 1985; 190:147–52.CrossrefGoogle Scholar

  • 9.

    Kasagi K, Kousaka T, Higuchi K, Iida Y, Misaki T, Alam MS, et al. Clinical significance of measurements of antithyroid antibodies in the diagnosis of Hashimoto's thyroiditis: comparison with histological findings. Thyroid 1996; 6:445–50.CrossrefGoogle Scholar

  • 10.

    Takamatsu J, Yoshida S, Yokozawa T, Hirai K, Kuma K, Ohsawa N, et al. Correlation of antithyroglobulin and antithyroid-peroxidase antibody profiles with clinical and ultrasound characteristics of chronic thyroiditis. Thyroid 1998; 8:1101–6.CrossrefGoogle Scholar

  • 11.

    Lindberg B, Svensson J, Ericsson UB, Nilsson P, Svenonius E, Ivarsson SA. Comparison of some different methods for analysis of thyroid autoantibodies: importance of thyroglobulin autoantibodies. Thyroid 2001; 11:265–9.CrossrefGoogle Scholar

  • 12.

    Saboori AM, Rose NR, Yuhasz SC, Amzel LM, Burek CL. Peptides of human thyroglobulin reactive with sera of patients with autoimmune thyroid disease. J Immunol 1999; 63:6244–50.Google Scholar

  • 13.

    Rose NR, Burek CL. Autoantibodies to thyroglobulin in health and disease. Appl Biochem Biotechnol 2000; 83:245–51.CrossrefGoogle Scholar

  • 14.

    Feldt-Rasmussen U. Analytical and clinical performance goals for testing autoantibodies to thyroperoxidase, thyroglobulin, and thyrotropin receptor. Clin Chem 1996; 42:160–3.Google Scholar

  • 15.

    Tozzoli R, Bizzaro N, Tonutti E, Pradella M, Manoni F, Villalta D, et al. Immunoassay of anti-thyroid autoantibodies: high analytical variability in second generation methods. Clin Chem Lab Med 2002; 40:568–73.CrossrefGoogle Scholar

  • 16.

    Wood WG, Hanke R. Comparability of method results and performance in a national external quality assessment scheme between 1993 and 2003 using thyroid associated antibodies as examples. Clin Lab 2004; 50:209–21.Google Scholar

  • 17.

    Gonzales C, Hernando M, Cava F, Herrero E, Garcia-Diez LC, Navajo JA, et al. Biological variability of thyroid autoantibodies in clinically and biochemically stable patients with autoimmune thyroid disease. J Clin Lab Anal 2002; 16:37–9.CrossrefGoogle Scholar

  • 18.

    Tozzoli R, Villalta D, Bizzaro N, Tonutti E, Manoni F. Laboratory diagnosis of autoimmune thyroid disease. Recenti Prog Med 2001; 92:609–17.Google Scholar

  • 19.

    Pandey A, Mann M. Proteomics to study genes and genomes. Nature 2000; 405:837–46.CrossrefGoogle Scholar

  • 20.

    Nolan JP, Mandy FF. Suspension array technology: new tools for gene and protein analysis. Cell Mol Biol 2001; 47:1241–56.Google Scholar

  • 21.

    Robinson WH, Steinman L, Utz PJ. Proteomics technology for the study of autoimmune disease. Arthritis Rheum 2002; 46:885–93.CrossrefGoogle Scholar

  • 22.

    Robinson WH, DiGennaro C, Hueber W, Haab BB, Kamachi M, Dean EJ, et al. Autoantigen microarrays for multiplex characterization of autoantibody responses. Nat Med 2002; 8:295–301.CrossrefGoogle Scholar

  • 23.

    Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettmann JR Jr. Advanced multiplexed analysis with FluoMetrix system. Clin Chem 1997; 43:1749–56.Google Scholar

  • 24.

    Rouquette AM, Desgruelles C, Laroche P. Evaluation of the new multiplexed immunoassay, FIDIS, for simultaneous quantitative determination of antinuclear antibodies and comparison with conventional methods. Am J Clin Pathol 2003; 120:676–81.CrossrefGoogle Scholar

  • 25.

    Prentice LM, Phillips DI, Sarsero D, Beever J, McLachlan SM, Smith BR. Geographical distribution of subclinical autoimmune thyroid disease in Britain: a study using highly sensitive direct assays for autoantibodies to thyroglobulin and thyroid peroxidase. Acta Endocrinol (Copenh) 1990; 123:493–8.Google Scholar

  • 26.

    Vanderpump MP, Turnbridge WM, French JM, Appleton D, Bates D, Clark F, et al. The incidence of thyroid disorders in the community: a twenty-year follow-up of the Whickam Survey. Clin Endocrinol (Oxf) 1995; 43:55–68.CrossrefGoogle Scholar

  • 27.

    Gilmour J, Brownlee Y, Foster P, Geekie C, Kelly P, Robertson S, et al. The quantitative measurement of autoantibodies to thyroglobulin and thyroid peroxidase by automated microparticle based immunoassays in Hashimoto's disease, Graves' disease and follow-up study on postpartum thyroid disease. Clin Lab 2000; 46:57–61.Google Scholar

  • 28.

    Lavard L, Perrild H, Jacobsen BB, Hoier-Madsen M, Bendinelli G, Vitti P. Prevalence of thyroid peroxidase, thyroglobulin, and thyrotropin receptor antibodies in a long-term follow-up of juvenile Graves' disease. Autoimmunity 2000; 32:167–72.CrossrefGoogle Scholar

  • 29.

    Takamatsu J, Yoshida S, Yokozawa T, Hirai K, Kuma K, Ohsawa N, et al. Correlation of antithyroglobulin and antithyroid-peroxidase antibody profiles with clinical and ultrasound characteristics of chronic thyroiditis. Thyroid 1998; 8:1101–6.CrossrefGoogle Scholar

  • 30.

    Banga JP. Thyroid peroxidase: development in our understanding of its structure and the relevance of these findings to autoimmunity. Curr Opin Endocrinol Diab 1998; 5:275–81.CrossrefGoogle Scholar

  • 31.

    Guo J, Wang Y, Rapoport B, McLachlan S. Evidence for antigen presentation to sensitized T cells by thyroid peroxidase (TPO)-specific B cells in mice injected with fibroblasts coexpressing TPO and MHC class II. Clin Exp Immunol 2000; 119:38–46.CrossrefGoogle Scholar

  • 32.

    Nielsen CH, Hegedus L, Leslie RG. Autoantibodies in autoimmune thyroid disease promote immune complex formation with self antigens and increase B cell and CD4+ T cell proliferation in response to self antigens. Eur J Immunol 2004; 34:263–72.CrossrefGoogle Scholar

  • 33.

    McLachlan S, Rapoport B. Autoimmune response to the thyroid in humans: thyroid peroxidase — the common autoantigenic denominator. Int Rev Immunol 2000; 19:587–618.CrossrefGoogle Scholar

  • 34.

    Hueber W, Utz PJ, Steinman L, Robinson WH. Autoantibody profiling for the study and treatment of autoimmune disease. Arthritis Res 2002; 4:290–5.CrossrefGoogle Scholar

About the article

Corresponding author: Nicola Bizzaro, MD, Laboratory of Clinical Pathology, Ospedale Civile, 33028 Tolmezzo (UD), Italy Phone: +39-0433-488261


Received: 2005-12-05

Accepted: 2006-03-10

Published Online: 2006-06-16

Published in Print: 2006-07-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.137.

Export Citation

©2006 by Walter de Gruyter Berlin New York. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Gabriela Brenta, Mario Vaisman, José Augusto Sgarbi, Liliana Maria Bergoglio, Nathalia Carvalho de Andrada, Pedro Pineda Bravo, Ana Maria Orlandi, and Hans Graf
Arquivos Brasileiros de Endocrinologia & Metabologia, 2013, Volume 57, Number 4, Page 265
[3]
N. Charfi, G. Chabchoub, M. Mnif, N. Elleuch Bougacha, J. Jouida, H. Ayadi, and M. Abid
Pathologie Biologie, 2009, Volume 57, Number 5, Page 378
[4]
Luca Giovanella, Emanuela Toffalori, Renato Tozzoli, Marco Caputo, Luca Ceriani, and Frederik A. Verburg
Head & Neck, 2012, Volume 34, Number 10, Page 1369
[5]
Michelle Trevisin, Wendy Pollock, Wayne Dimech, and Judy Savige
Journal of Immunological Methods, 2008, Volume 336, Number 2, Page 104
[6]
Peter Lea, Edward Keystone, Sasi Mudumba, Anthony Kahama, Shi-Fa Ding, Jennifer Hansen, Azar A. Azad, Sihe Wang, and Deborah Weber
Clinical Reviews in Allergy & Immunology, 2011, Volume 41, Number 1, Page 20
[7]
R. Tozzoli, O. Barzilai, M. Ram, D. Villalta, N. Bizzaro, Y. Sherer, and Y. Shoenfeld
Autoimmunity Reviews, 2008, Volume 8, Number 2, Page 112
[9]
Renato Tozzoli
Clinical Chemistry and Laboratory Medicine, 2008, Volume 46, Number 5
[11]
Patrizio Caturegli, Hiroaki Kimura, Roberto Rocchi, and Noel R Rose
Current Opinion in Rheumatology, 2007, Volume 19, Number 1, Page 44
[12]
Nicola Bizzaro, Renato Tozzoli, and Yehuda Shoenfeld
Arthritis & Rheumatism, 2007, Volume 56, Number 6, Page 1736

Comments (0)

Please log in or register to comment.
Log in