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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Volume 44, Issue 8


Polymorphisms of the inflammatory system and risk of ischemic cerebrovascular events

Wolfgang Lalouschek / Martin Schillinger / Kety Hsieh
  • Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria
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/ Georg Endler
  • Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria
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/ Stefan Greisenegger / Rodrig Marculescu
  • Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria
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/ Wilfried Lang / Oswald Wagner
  • Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria
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/ Suzanne Cheng
  • Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, CA, United States of America
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/ Christine Mannhalter
  • Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria
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Published Online: 2006-07-31 | DOI: https://doi.org/10.1515/CCLM.2006.165


Background: Chronic and acute infections are associated with an increased risk of stroke. The inflammatory response can be influenced by functional polymorphisms in components of the immune system. We hypothesized that these polymorphisms may also modulate the risk of ischemic cerebrovascular events.

Methods: We determined the frequency of polymorphisms in tumor necrosis factor-α[(TNF-α) G(–376)A, G(–244)A, G(–238)A, G(–308)A], Toll-like receptor 4 [(TLR4) Gly299Asp and Thr399Ile], interleukin-1-receptor antagonist [(IL-1-RA) intron 2 variable-number tandem repeat], monocyte differentiation antigen CD14 receptor C(–260)T, and interleukin-6 [(IL-6) G(–174)C] genes in 404 patients with acute stroke or transient ischemic attack before the age of 60years and in 415 healthy individuals. We also tested for interactions between genotypes, recent febrile episodes and stroke risk.

Results: None of the polymorphisms was associated with an increased risk of stroke after adjustment for age and gender. Following multivariate adjustment, carriers of the TNF-α (–308)A allele, the IL-1-RA 2* allele or the IL-6 (–174)C allele appeared to have an increased risk of stroke in association with a febrile episode prior to strokes.

Conclusion: In our study none of the investigated polymorphisms of the inflammatory system was associated with the risk of acute cerebrovascular events before the age of 60years. However, post-hoc analyses indicate that some polymorphisms seem to contribute to the risk of stroke in combination with fever.

Clin Chem Lab Med 2006;44:918–23.

Keywords: infection; inflammation; interleukins; polymorphism; stroke; tumor necrosis factor


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About the article

Corresponding author: Prof. Wolfgang Lalouschek, MD, University Clinic of Neurology, Clinical Department of Clinical Neurology, Waehringer Guertel 18-20, 1097 Vienna, Austria Phone: +43-1-40400-3117, Fax: +43-1-40400-3141

Received: 2006-01-26

Accepted: 2006-04-21

Published Online: 2006-07-31

Published in Print: 2006-08-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 8, Pages 918–923, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.165.

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