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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 44, Issue 9

Issues

Lower expression of the α2,3-sialylated fibronectin glycoform and appearance of the asialo-fibronectin glycoform are associated with high concentrations of fibronectin in human seminal plasma with abnormal semen parameters

Iwona Kątnik-Prastowska / Ewa M. Kratz / Ricardo Faundez / Anna Chełmońska-Soyta
  • Laboratory of Reproductive Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
  • Other articles by this author:
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Published Online: 2006-09-08 | DOI: https://doi.org/10.1515/CCLM.2006.193

Abstract

Background: Sialic acid isoforms expressed on glycoconjugates are known to be involved in different biological functions. The aim of this study was to compare α2,3- and/or α2,6-linked sialic acid expression on fibronectin in the seminal plasma of male partners from infertile couples. The data obtained were analyzed in relation to normal and abnormal semen parameters, as well as to fibronectin concentrations.

Methods: Fibronectin concentrations were determined by ELISA using a specific monoclonal antibody. The relative degree of sialic acid linked to fibronectin glycans either by α2,3 or α2,6 isomeric linkage was estimated by lectin-fibronectin ELISA utilizing lectins from Maackia amurensis and Sambucus nigra, respectively.

Results: High seminal fibronectin concentration was more frequently associated with abnormal semen parameters. The glycans of seminal plasma fibronectin, in contrast to blood plasma fibronectin, contained sialic acid more frequently attached by α2,3 than by α2,6 isomeric linkage. The relative amounts of both α2,3- and α2,6-linked sialic acid fibronectin isoforms were lower in the seminal plasma of men suspected of infertility.

Conclusions: Seminal fibronectin showed an oncofetal type of sialylation and the distribution of hypo- and asialylated fibronectin glycoforms were associated with abnormal semen parameters and with high concentrations of fibronectin.

Clin Chem Lab Med 2006;44:1119–25.

Keywords: fibrin-heparin fibronectin domain; fibronectin glycoforms; infertility; seminal plasma; sialylation

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About the article

Corresponding author: Prof. Dr. Iwona Kątnik-Prastowska, Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345 Wrocław, Poland Phone: +48-71-3281648, Fax: +48-71-3281649,


Received: 2006-03-24

Accepted: 2006-06-02

Published Online: 2006-09-08

Published in Print: 2006-09-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 44, Issue 9, Pages 1119–1125, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2006.193.

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