Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

Online
ISSN
1437-4331
See all formats and pricing

 


Select Volume and Issue

Issues

30,00 € / $42.00 / £23.00

Get Access to Full Text

Frequency of two human glutathione-S-transferase omega-1 polymorphisms (E155 deletion and E208K) in Ovambo and Japanese populations using the PCR-based genotyping method

Junko Fujihara1 / Takashi Kunito2 / Haruo Takeshita3

1Department of Legal Medicine, Shimane University School of Medicine, Izumo, Shimane, Japan

2Department of Environmental Sciences, Faculty of Science, Shinshu University, Matsumoto, Nagano, Japan

3Department of Legal Medicine, Shimane University School of Medicine, Izumo, Shimane, Japan

Corresponding author: Haruo Takeshita, MD, PhD, Department of Legal Medicine, Shimane University School of Medicine, 89-1 Enya, Izumo, Shimane, Japan Phone: +81-853-20-2156, Fax: +81-853-20-2155,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 45, Issue 5, Pages 621–624, ISSN (Online) 14346621, ISSN (Print) 14374331, DOI: https://doi.org/10.1515/CCLM.2007.128, May 2007

Publication History

Received:
2006-12-25
Accepted:
2007-02-11
Published Online:
2007-05-07

Abstract

Background: Human glutathione S-transferase omega-1 (hGSTO1) has monomethylarsonate (MMAv) reductase activity. Recent study suggests that two polymorphisms (E155 deletion and E208K) in hGSTO1 can be related to inter-individual variations in inorganic arsenic metabolism. As useful PCR-based genotyping methods for these hGSTO1 polymorphisms are not available and data on hGSTO1 polymorphism in African and Asian populations are insufficient, the aim of the present study was to develop a PCR-based genotyping method for E155del and E208K, and to investigate the allele frequencies of these two polymorphisms in Ovambo and Japanese populations.

Methods: The E155del and E208K polymorphisms were detected using confronting two-pair primers analysis and PCR-restriction fragment length polymorphism, respectively.

Results: Allele frequencies for the hGSTO1 polymorphisms were investigated in Ovambo (n=144) and Japanese (n=144) populations. For E155del, the mutation frequency in Ovambo and Japanese subjects was 0.000 and 0.017, respectively, similar to those for other populations. As for E208K polymorphism, no mutation allele was found in Ovambo or Japanese subjects.

Conclusions: The present study developed a PCR-based hGSTO1 genotyping method that could be applied to a large number of individuals at most locations.

Clin Chem Lab Med 2007;45:621–4.

Keywords: arsenic metabolism; glutathione S-transferase omega-1; Japanese; monomethylarsonate reductase; Ovambos; polymorphism

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
S. Sanguansin, S. Petmitr, P. O-charoenrat, and W. Pongstaporn
Molecular Biology Reports, 2012, Volume 39, Number 12, Page 10915
[2]
J. Plantamura, F. Dorandeu, P. Burnat, and C. Renard
Annales Pharmaceutiques Françaises, 2011, Volume 69, Number 4, Page 196
[3]
Chi-Jung Chung, Yeong-Shiau Pu, Chien-Tien Su, Chao-Yuan Huang, and Yu-Mei Hsueh
Science of The Total Environment, 2011, Volume 409, Number 3, Page 465
[4]
Junko Fujihara, Yoshimi Fujii, Tetsuro Agusa, Takashi Kunito, Toshihiro Yasuda, Tamami Moritani, and Haruo Takeshita
Toxicology and Applied Pharmacology, 2009, Volume 234, Number 1, Page 41
[5]
S. Piacentini, R. Polimanti, B. Moscatelli, M.A. Re, R. Fuciarelli, D. Manfellotto, and M. Fuciarelli
Annals of Human Biology, 2010, Volume 37, Number 3, Page 427
[6]
Alba Hernández and Ricard Marcos
Pharmacogenomics, 2008, Volume 9, Number 8, Page 1113
[7]
Junko Fujihara, Toshihiro Yasuda, Hideaki Kato, Isao Yuasa, Arturo Panduro, Takashi Kunito, and Haruo Takeshita
Archives of Toxicology, 2011, Volume 85, Number 2, Page 119
[8]
Haruo Takeshita, Junko Fujihara, Hisakazu Takastuka, Tetsuro Agusa, Toshihiro Yasuda, and Takashi Kunito
Clinical and Experimental Pharmacology and Physiology, 2009, Volume 36, Number 3, Page 283

Comments (0)

Please log in or register to comment.