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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 45, Issue 7

Issues

Effect of 13-cis-retinoic acid on the genetic expression profile of human umbilical vein endothelial cells (HUVECs) determined by microarray

Juliana Salazar
  • 1Unitat de Recerca de Lípids i Arteriosclerosi, Institut de la Recerca en Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mercedes Camacho
  • 2Labaratori de Mediadors de la Inflamació, Institut de Recerca del Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lluís Masana
  • 3Unitat de Recerca de Lípids i Arteriosclerosi, Institut de la Recerca en Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Luis Vila
  • 4Labaratori de Mediadors de la Inflamació, Institut de Recerca del Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Josep Ribalta
  • 5Unitat de Recerca de Lípids i Arteriosclerosi, Institut de la Recerca en Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2007-07-08 | DOI: https://doi.org/10.1515/CCLM.2007.139

Abstract

Background: Treatment with isotretinoin (13-cis-retinoic acid; 13-cis RA) has been associated with hypertriglyceridemia and low levels of high-density lipoprotein (HDL)-cholesterol, which are well-established cardiovascular risk factors. The development of hypertriglyceridemia secondary to this treatment could predispose to the metabolic syndrome. Hence, because of the risk of atherosclerosis associated with this treatment and to help unravel the underlying mechanisms involved, we studied the effect of 13-cis RA on genetic expression in endothelial cells.

Methods: Total RNA was isolated from human umbilical vein endothelial cells (HUVECs) treated without 13-cis RA (control) or incubated with 13-cis RA (1 μM) for 6 and 24 h. Microarray analysis (Affymetrix U133A array) with >22,000 probes was used with real time RT-PCR of a series of genes selected because of their involvement in the RA signaling pathway.

Results: The treatment of endothelial cells with 13-cis RA produced significant changes in the expression of genes implicated in cell adhesion and in lipid metabolism processes, specifically in the clearance of lipoprotein remnant particles and in HDL metabolism.

Conclusions: Isotretinoin in the endothelium has an exacerbating effect on atherosclerosis risk factors. This is especially relevant in the regulation of expression of genes involved in the processes of cell adhesion and lipid metabolism.

Clin Chem Lab Med 2007;45:829–34.

Keywords: cell adhesion; 13-cis-retinoic acid; high-density lipoprotein; human umbilical vein endothelial cell (HUVEC); isotretinoin

About the article

Corresponding author: Josep Ribalta, PhD, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, C/ Sant Llorenç, 21, 43201 Reus, Spain Phone: +34-977-759390, Fax: +34-977-759322


Received: 2006-11-16

Accepted: 2007-01-15

Published Online: 2007-07-08

Published in Print: 2007-07-01


Citation Information: Clinical Chemical Laboratory Medicine, Volume 45, Issue 7, Pages 829–834, ISSN (Online) 14374331, ISSN (Print) 14346621, DOI: https://doi.org/10.1515/CCLM.2007.139.

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