Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 46, Issue 1 (Jan 2008)

Issues

Functional polymorphisms in the promoter of the matrix metalloproteinase-9 (MMP-9) gene are not linked with significant plasma MMP-9 variations in healthy subjects

Caroline Demacq
  • 1Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Vivian B. Vasconcellos
  • 2Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Andrea M. Marcaccini
  • 3Department of Morphology, Estomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Raquel F. Gerlach
  • 4Department of Morphology, Estomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Wilson A. Silva Jr.
  • 5Department of Genetics and Center for Cell Based Therapy, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jose E. Tanus-Santos
  • 6Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Background: Matrix metalloproteinase-9 (MMP-9) is involved in the degradation of the extracellular matrix during physiological and pathological processes. Two functional polymorphisms [C–1562T and microsatellite (CA)13–25] in the promoter region of the MMP-9 gene have been associated with several diseases. The aim of this study was to examine whether these MMP-9 polymorphisms and haplotypes are linked with plasma MMP-9 variations in healthy subjects.

Methods: We studied 177 healthy male white volunteers (age range 20–55 years) who were non-smokers and not taking any medication. Genomic DNA was extracted from whole blood and genotypes for the C–1562T and the microsatellite (CA)n polymorphisms were determined. MMP-9 levels were measured in plasma samples by gelatin zymography.

Results: The frequency of the alleles C and T for the C–1562T polymorphism were 90% and 10%, respectively. The frequency of the alleles with less than 21 CA repeats (L) and with 21 repeats or higher (H) were 47% and 53%, respectively. We found no differences in plasma MMP-9 levels among the genotype groups or among different haplotypes (all p>0.05).

Conclusions: These findings suggest that functional polymorphisms in the promoter of the MMP-9 gene are not linked with significant plasma MMP-9 variations in healthy subjects.

Clin Chem Lab Med 2008;46:57–63.

Keywords: haplotypes; metalloproteinase-9; polymorphism; zymography

About the article

Corresponding author: Jose Eduardo Tanus-Santos, MD, PhD, Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, Brazil Phone: +55-16-3602-3163, Fax: +55-16-3633-2301,


Received: 2007-07-10

Accepted: 2007-09-03

Published in Print: 2008-01-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.014.

Export Citation

©2008 by Walter de Gruyter Berlin New York. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sonja Stojanovic, Bojana Stamenkovic, Tatjana Jevtovic Stoimenov, Jovan Nedovic, Valentina Zivkovic, Milena Despotovic, and Dusica Pavlovic
Clinical Rheumatology, 2017, Volume 36, Number 7, Page 1479
[2]
Yuanyuan Xu, Yan Wang, Jixin Zhi, Lichun Qi, Tong Zhang, and Xueqi Li
BMC Pharmacology and Toxicology, 2017, Volume 18, Number 1
[3]
Trine B. Opstad, Harald Arnesen, Alf Å. Pettersen, Ingebjørg Seljeflot, and Michael Lipinski
PLoS ONE, 2014, Volume 9, Number 9, Page e106816
[4]
Zohreh Rahimi, Zahra Abdan, Ziba Rahimi, Nazanin Razazian, Hadis Shiri, Asad Vaisi-Raygani, Ebrahim Shakiba, Mahmood Vessal, and Mohammad-Taher Moradi
Immunological Investigations, 2016, Volume 45, Number 6, Page 543
[5]
Deepal Haresh Ajmera, Pradeep Singh, Ying Zhu, Wenyang Li, and Jinlin Song
SpringerPlus, 2016, Volume 5, Number 1
[6]
Sara Mohseni, Tahereh Tohidi Moghadam, Bahareh Dabirmanesh, Safoura Jabbari, and Khosro Khajeh
Biosensors and Bioelectronics, 2016, Volume 81, Page 510
[7]
Natalia A. Juiz, Nelly M. Cayo, Marianela Burgos, Miriam E. Salvo, Julio R. Nasser, Jacqueline Búa, Silvia A. Longhi, and Alejandro G. Schijman
Journal of Infectious Diseases, 2016, Volume 213, Number 8, Page 1299
[8]
Xiao Wang and Lei-zhi Shi
Journal of Zhejiang University SCIENCE B, 2014, Volume 15, Number 3, Page 256
[9]
Diego Robles Mazzotti, Cristiane Carvalho Singulane, Vanessa Kiyomi Ota, Thiago Potrich Rodrigues, Tatiane Katsue Furuya, Fernando José de Souza, Bruna Grassiela Cordeiro, Camilla Magalhães de Oliveira Amaral, Elizabeth Suchi Chen, Anielli Jacomini, Marilia de Arruda Cardoso Smith, and Bianca Borsatto-Galera
Gene, 2014, Volume 535, Number 2, Page 370
[10]
Zohreh Rahimi, Ziba Rahimi, Maria Omidi Shahsavandi, Kazem Bidoki, and Mansour Rezaei
Biomarkers in Medicine, 2013, Volume 7, Number 1, Page 93
[11]
Bernardo P. Marson, Riccardo Lacchini, Vanessa Belo, Silvia G. Mattos, Bartira P. da Costa, Carlos E. Poli-de-Figueiredo, and Jose E. Tanus-Santos
Clinica Chimica Acta, 2012, Volume 414, Page 46
[12]
Alisson Martins-Oliveira, Flavia M. Gonçalves, Jose G. Speciali, Vanessa Fontana, Tatiane C. Izidoro-Toledo, Vanessa A. Belo, Fabiola Dach, and Jose E. Tanus-Santos
Journal of Neuroimmunology, 2012, Volume 252, Number 1-2, Page 89
[13]
Karla Simone da Silva Fernandes, Doralina Guimaraes Brum, Ana Carolina Palei, Valeria Cristina Sandrim, Carlos Tostes Guerreiro, José Eduardo Tanus-Santos, and Amilton Antunes Barreira
Journal of Neuroimmunology, 2012, Volume 249, Number 1-2, Page 56
[14]
A Rietz and JP Spiers
British Journal of Pharmacology, 2012, Volume 166, Number 4, Page 1225
[15]
Bernardo P. Marson, Carlos E. Poli de Figueiredo, and Jose E. Tanus-Santos
Basic & Clinical Pharmacology & Toxicology, 2012, Volume 110, Number 5, Page 409
[16]
Marcelo Rizzatti Luizon and Vanessa de Almeida Belo
Biomarkers, 2012, Volume 17, Number 3, Page 286
[17]
Ingrid F. Metzger, Marcelo R. Luizon, Riccardo Lacchini, and Jose Eduardo Tanus-Santos
DNA and Cell Biology, 2012, Volume 31, Number 4, Page 504
[18]
V A Belo, D C Souza-Costa, M R Luizon, C M M Lanna, P C Carneiro, T C Izidoro-Toledo, K C Ferraz, and J E Tanus-Santos
International Journal of Obesity, 2012, Volume 36, Number 1, Page 69
[19]
Ingrid F. Metzger, Valéria C. Sandrim, and Jose E. Tanus-Santos
Molecular and Cellular Biochemistry, 2012, Volume 360, Number 1-2, Page 393
[20]
Yun Jong Lee, Seong Wook Kang, Han Joo Baek, Hyo Jin Choi, Young Deok Bae, Eun Ha Kang, Eun Young Lee, Eun Bong Lee, and Yeong Wook Song
Human Immunology, 2010, Volume 71, Number 7, Page 717
[21]
Yun Jong Lee, Mijung Woo, Jung-Hyun Nam, Jinah Baek, Churl Hyun Im, Eun Young Lee, Eun Bong Lee, Kyung Sook Park, and Yeong Wook Song
Human Immunology, 2008, Volume 69, Number 6, Page 374
[22]
Caroline Demacq, Ingrid F. Metzger, Raquel F. Gerlach, and Jose E. Tanus-Santos
Clinica Chimica Acta, 2008, Volume 394, Number 1-2, Page 72
[23]
Riccardo Lacchini, Anna L.B. Jacob-Ferreira, Marcelo R. Luizon, Fernanda B. Coeli, Tatiane C. Izidoro-Toledo, Sandra Gasparini, Maria C. Ferreira-Sae, Roberto Schreiber, Wilson Nadruz, and Jose E. Tanus-Santos
Clinica Chimica Acta, 2010, Volume 411, Number 23-24, Page 1940
[24]
Ana C.T. Palei, Valeria C. Sandrim, Geraldo Duarte, Ricardo C. Cavalli, Raquel F. Gerlach, and Jose E. Tanus-Santos
Clinica Chimica Acta, 2010, Volume 411, Number 11-12, Page 874
[25]
Ying Wang, Ying Su, Ying Xu, Shang-Ha Pan, and Guo-Dong Liu
Biochemical and Biophysical Research Communications, 2010, Volume 391, Number 1, Page 113
[26]
Diana M. Isaza-Guzmán, Carolina Arias-Osorio, María C. Martínez-Pabón, and Sergio I. Tobón-Arroyave
Archives of Oral Biology, 2011, Volume 56, Number 4, Page 401
[27]
Molly C. Madden, William C. Byrnes, Jacob A. Lebin, Matthew E. Batliner, and David L. Allen
European Journal of Applied Physiology, 2011, Volume 111, Number 8, Page 1795
[28]
A C T Palei, V C Sandrim, L M Amaral, J S R Machado, R C Cavalli, R Lacchini, G Duarte, and J E Tanus-Santos
The Pharmacogenomics Journal, 2012, Volume 12, Number 6, Page 489
[29]
Riccardo Lacchini, Ingrid F. Metzger, Marcelo Luizon, Marília Ishizawa, and Jose E. Tanus-Santos
DNA and Cell Biology, 2010, Volume 29, Number 11, Page 649
[30]
Semon Wu, Lung-An Hsu, Ming-Sheng Teng, Jeng Feng Lin, Hsien-Hsun Chang, Pi-Yueh Chang, Chiao-Feng Hu, and Yu-Lin Ko
Clinical Chemistry and Laboratory Medicine, 2010, Volume 48, Number 4
[31]
Jelena Basic, Dusica Pavlovic, Tatjana Jevtovic-Stoimenov, Jelena Vojinovic, Gordana Susic, Ivana Stojanovic, Gordana Kocic, Vuk Milosevic, Tatjana Cvetkovic, Milena Marinkovic, and Andrej Veljkovic
Journal of Physiology and Biochemistry, 2010, Volume 66, Number 2, Page 173
[32]
C Demacq, V B Vasconcellos, A M Marcaccini, R F Gerlach, A A Machado, and J E Tanus-Santos
The Pharmacogenomics Journal, 2009, Volume 9, Number 4, Page 265

Comments (0)

Please log in or register to comment.
Log in