Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

See all formats and pricing
Volume 46, Issue 11 (Nov 2008)


Rapid sample preparation and simultaneous quantitation of prostaglandins and lipoxygenase derived fatty acid metabolites by liquid chromatography-mass spectrometry from small sample volumes

Ines Unterwurzacher
  • 1Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University, Innsbruck, Austria and Biocrates Life Sciences AG, Innsbruck, Austria
/ Therese Koal
  • 2Biocrates Life Sciences AG, Innsbruck, Austria
/ Guenther K. Bonn
  • 3Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University, Innsbruck, Austria
/ Klaus M. Weinberger
  • 4Biocrates Life Sciences AG, Innsbruck, Austria
/ Steven L. Ramsay
  • Current address: CSL Limited, 45 Poplar Road, Parkville, VIC 3052, Australia.
    5Biocrates Life Sciences AG, Innsbruck, Austria
Published Online: 2008-10-09 | DOI: https://doi.org/10.1515/CCLM.2008.323


Background: Fatty acid metabolites play a key role in numerous physiological and pathological processes. A rapid liquid chromatography-mass spectrometry assay for the simultaneous determination of prostanoids, isoprostane and lipoxygenase (LOX) derived fatty acid metabolites in a small biological sample of only 20 μL was developed.

Methods: Human plasma samples were applied to a filter spot, extracted without prior derivatization and analyzed within 13 min. Detection of metabolites was performed on a triple quadrupole mass spectrometer in negative multiple-reaction monitoring detection mode. Application of this assay to various biological matrices was performed.

Results: The validated assay was linear over the concentration range of 5–500 nmol/L for prostanoids and isoprostane, 50–5000 nmol/L for LOX-derived metabolites and 400–40,000 nmol/L for fatty acids. Limits of quantitation were 0.4–233 nmol/L, depending on the metabolite. Plasma samples from diabetic patients and controls showed significant increases in (±)9-HODE and 15(S)-HETE with p-values of 0.019 and 0.024, respectively.

Conclusions: The small amount of 20 μL sample volume used in this assay and the demonstrated application to various sample types makes it an ideal routine analysis method for fatty acid metabolites. The resulting values for LOX-derived metabolites in diabetes mellitus type 2 samples support earlier findings about the role of lipid oxidation products in diabetes.

Clin Chem Lab Med 2008;46:1589–97.

Keywords: diabetes; fatty acid metabolites; liquid chromatography-mass spectrometry (LC-MS/MS); lipid oxidation products; prostaglandins

About the article

Corresponding author: I. Unterwurzacher, Biocrates Life Sciences AG, Innrain 66, 6020 Innsbruck, Austria Phone: +43-5125798234235, Fax: +43-5125798234270,

Received: 2008-05-29

Accepted: 2008-08-01

Published Online: 2008-10-09

Published in Print: 2008-11-01

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.323. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Peter M. Abuja, Friederike Ehrhart, Uwe Schoen, Tomm Schmidt, Frank Stracke, Guido Dallmann, Torben Friedrich, Heiko Zimmermann, and Kurt Zatloukal
Journal of Proteome Research, 2015, Page 150611100320000
Ali Aghazadeh-Habashi, Waheed Asghar, and Fakhreddin Jamali
Journal of Pharmaceutical and Biomedical Analysis, 2015, Volume 110, Page 12
Antje Damms-Machado, Suparna Mitra, Asja E. Schollenberger, Klaus Michael Kramer, Tobias Meile, Alfred Königsrainer, Daniel H. Huson, and Stephan C. Bischoff
BioMed Research International, 2015, Volume 2015, Page 1
C. Morgantini, A. Natali, B. Boldrini, S. Imaizumi, M. Navab, A. M. Fogelman, E. Ferrannini, and S. T. Reddy
Diabetes, 2011, Volume 60, Number 10, Page 2617
Xinxing Tang, Yuan Gu, Jihua Nie, Saijun Fan, and Chang Wang
Journal of Liquid Chromatography & Related Technologies, 2014, Volume 37, Number 7, Page 951
Jafar Sadik B. Shaik, Tricia M. Miller, Steven H. Graham, Mioara D. Manole, and Samuel M. Poloyac
Journal of Chromatography B, 2014, Volume 945-946, Page 207
Mahin Moghaddami, Enzo Ranieri, Michael James, Janice Fletcher, and Leslie G. Cleland
Mediators of Inflammation, 2013, Volume 2013, Page 1
Xinxing Tang, Mingchen Zheng, Yuanyuan Zhang, Saijun Fan, and Chang Wang
Metabolomics, 2013, Volume 9, Number 4, Page 853
Bruno Casetta, Mariangela Longini, Fabrizio Proietti, Serafina Perrone, and Giuseppe Buonocore
Journal of Maternal-Fetal and Neonatal Medicine, 2012, Volume 25, Number S1, Page 114
Sandra Rinne Dahl, Charlotte Ramstad Kleiveland, Moustapha Kassem, Tor Lea, Elsa Lundanes, and Tyge Greibrokk
Journal of Chromatography A, 2009, Volume 1216, Number 22, Page 4648
Susen Becker, Linda Kortz, Christin Helmschrodt, Joachim Thiery, and Uta Ceglarek
Journal of Chromatography B, 2012, Volume 883-884, Page 68
Raquel Martín-Venegas, Rosario Casillas, Olga Jáuregui, and Juan José Moreno
Journal of Pharmaceutical and Biomedical Analysis, 2011, Volume 56, Number 5, Page 976
C. Ferreiro-Vera, J.M. Mata-Granados, F. Priego-Capote, and M.D. Luque de Castro
Journal of Chromatography A, 2011, Volume 1218, Number 20, Page 2848
Journal of Mass Spectrometry, 2009, Volume 44, Number 5, Page 848

Comments (0)

Please log in or register to comment.
Log in