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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

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1437-4331
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Volume 46, Issue 2 (Feb 2008)

Issues

Assay-dependent variability of serum insulin levels during oral glucose tolerance test: influence on reference intervals for insulin and on cut-off values for insulin sensitivity indices

Maria Werner
  • 1Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Leipzig, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anke Tönjes
  • 2III Medical Department, University of Leipzig, Leipzig, Germany and Coordination Centre for Clinical Trials, University of Leipzig, Leipzig, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Michael Stumvoll / Joachim Thiery
  • 4Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Leipzig, Germany
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  • De Gruyter OnlineGoogle Scholar
/ Jürgen Kratzsch
  • 5Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Leipzig, Germany
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Published Online: 2007-12-13 | DOI: https://doi.org/10.1515/CCLM.2008.020

Abstract

Background: The oral glucose tolerance test (oGTT) is the most common method to estimate indices of insulin sensitivity in clinical as well as in epidemiological studies. The aim of this study was to investigate the variability of insulin levels for reference intervals and of insulin sensitivity indices during oGTT by three different insulin immunoassays.

Methods: Serum insulin levels were determined during oGTT of randomly selected adult subjects (total n=101, with n=68 with normal glucose tolerance) by the LIAISON (LIA), the Elecsys (EL) and the AutoDELFIA (AD) method.

Results: Sensitivity and precision of all three insulin assays were comparable. Insulin levels demonstrated a close (p<0.001) interassay correlation (LIA vs. EL: r=0.987, LIA vs. AD: r=0.966, EL vs. AD: r=0.965). Insulin levels and insulin sensitivity indices (n=68) of the AD method were significantly different compared to the LIA or EL assay (p<0.001). Basal insulin levels demonstrated an assay-dependent significant body mass index (BMI) dependency (LIA: r=0.24, p=0.05; AD: r=0.44, p<0.001; EL: r=0.36, p<0.01) in the regression analysis. At 120 min post glucose, gender was the relevant influencing factor on insulin levels of the LIA (r=0.40, p<0.001), AD (r=0.37, p<0.01) and EL (r=0.40 p<0.001) method.

Conclusions: We conclude that assay-dependent reference intervals should be used for the clinical interpretation of insulin levels from oGTT and of insulin sensitivity indices. Moreover, BMI and gender have to be considered as influencing factors.

Clin Chem Lab Med 2008;46:240–6.

Keywords: evaluation; glucose tolerance test; insulin measurement; insulin sensitivity indices; reference values

About the article

Corresponding author: Maria Werner, PhD, Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Paul-List-Str. 13-15, 04103 Leipzig, Germany Phone: +49-341-9722409,


Received: 2007-07-05

Accepted: 2007-09-06

Published Online: 2007-12-13

Published in Print: 2008-02-01


Citation Information: Clinical Chemical Laboratory Medicine, ISSN (Online) 14374331, ISSN (Print) 14346621, DOI: https://doi.org/10.1515/CCLM.2008.020.

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©2008 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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