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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Online
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1437-4331
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Volume 46, Issue 3 (Mar 2008)

Issues

Development of des-γ-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer

Isao Yamaguchi
  • 1New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Kenji Nakamura
  • 2New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Hiromichi Kitano
  • 3New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Yoshie Masuda
  • 4New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Futoshi Kanke
  • 5New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Shinzo Kobatake
  • 6New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
/ Shinji Satomura
  • 7New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan
Published Online: 2008-02-06 | DOI: https://doi.org/10.1515/CCLM.2008.078

Abstract

Background: Des-γ-carboxy prothrombin (DCP), Lens culinaris agglutinin-reactive α-fetoprotein ratio (AFP-L3) and total α-fetoprotein (AFP) are tumor markers useful for diagnosing and determining the prognosis of hepatocellular carcinoma (HCC). There is a real need for measurement of these three markers on a one-assay platform.

Methods: A method of DCP measurement in human serum was developed using liquid binding assay (LBA), which enables rapid antigen-antibody reaction and bound/free separation on the LiBASys clinical analyzer.

Results: The dilution curve for DCP was linear up to 500 ng/mL. The limit of detection of DCP concentration was 0.5 ng/mL. Intra- and inter-assay coefficients of variation of DCP were 0.7%–2.4% and 2.2%–6.5%, respectively. This method was free from interference by hemoglobin, bilirubin, ditaurobilirubin, intrafat, ascorbate, galactose, glucose and rheumatoid factor. The analytical recoveries of DCP added to serum were 91.7%–108.2%. DCP concentration measured with the LBA method was linear and was significantly correlated with that measured with the ELISA method.

Conclusions: The LiBASys clinical analyzer made possible measurement of the complementary tumor markers, HCC, total AFP, AFP-L3 and DCP.

Clin Chem Lab Med 2008;46:411–6.

Keywords: des-γ-carboxy-prothrombin; hepatocellular carcinoma; Lens culinaris agglutinin-reactive α-fetoprotein ratio (AFP-L3); LiBASys; total α-fetoprotein (AFP)

About the article

Corresponding author: Isao Yamaguchi, New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries, Ltd., 6-1 Takada-cho, Amagasaki, Hyogo, 661-0963, Japan Phone: +81-6-6499-9109, Fax: +81-6-6499-1524,


Received: 2007-09-10

Accepted: 2007-11-23

Published Online: 2008-02-06

Published in Print: 2008-03-01


Citation Information: Clinical Chemical Laboratory Medicine, ISSN (Online) 14374331, ISSN (Print) 14346621, DOI: https://doi.org/10.1515/CCLM.2008.078. Export Citation

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