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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
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1437-4331
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Volume 46, Issue 4 (Apr 2008)

Issues

Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?

Yun Lu
  • 1Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Li-Qun Wu
  • 2Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shou-Guang Wang
  • 3Center of Cell and Molecular Pathology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Zhen-Hua Lv
  • 4Center of Cell and Molecular Pathology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bing Han
  • 5Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-02-26 | DOI: https://doi.org/10.1515/CCLM.2008.094

Abstract

Background: The aim of this study was to detect the expression of apoptosis factor caspase-3 in transferred HepG2 cells and provide feasible evaluation of the treatment for primary liver cancer with gene methods.

Methods: The pcDNA4C-LIGHT cDNA was extracted from Escherichia coli JM-109; then, the pcDNA4C-LIGHT cDNA was transferred into the HepG2 cells by a cationic liposome mediated method. Meanwhile, the blank group was established as the control group and the HepG2 cells were collected after transfection at 12 h, 24 h, 48 h, 3 days and 5 days. The expression of caspase-3 was identified in the supernatants by ELISA. A standard curve was generated for the set of samples assayed. Statistical significance was analyzed by SPSS.

Results: The quantity of caspase-3 protein was the greatest at 48 h and the least on day 5. The secretion of caspase-3 did not increase in the control group. The coefficient of correlation was equal to 0.9986 and had evident significance.

Conclusions: The pcDNA4C-LIGHT was effectively transfected in human HepG2 cells mediated by liposome. The expression of caspase-3 increased in the transfected group. This study provides necessary theoretic support for the treatment of liver cancer with gene methods.

Clin Chem Lab Med 2008;46:470–4.

Keywords: caspase-3; hepatocellular carcinoma; HepG2 cell; LIGHT gene; transfection

About the article

Corresponding author: Yun Lu, Associate Professor, Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, No. 16 Jiangsu Rd., Qingdao 266003, Shandong Province, China Phone: +86-532-82911369, Fax: +86-532-82911999,


Received: 2007-10-20

Accepted: 2007-11-30

Published Online: 2008-02-26

Published in Print: 2008-04-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 14374331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.094.

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©2008 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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