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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

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Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 46, Issue 6

Issues

Urinary fructose-1,6-bisphosphatase activity as a marker of the damage to the renal proximal tubules in children with idiopathic nephrotic syndrome

Alina Kępka
  • 1Department of Laboratory Diagnostics of the Institute “Pomnik-Centrum Zdrowia Dziecka”, Warsaw, Poland
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/ Sławomir Dariusz Szajda
  • 2Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland
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/ Anna Stypułkowska
  • 3Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland
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/ Napoleon Waszkiewicz / Anna Jankowska / Sylwia Chojnowska
  • 6Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland
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/ Krzysztof Zwierz
  • 7Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland
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Abstract

Background: Disturbances in the function of renal proximal tubules increase the activity of several enzymes in urine. Among them is fructose-1,6-bisphosphatase (FBP-1), the key enzyme of gluconeogenesis normally present in the renal convoluted, and to smaller degree, proximal renal tubular cells cytosol. FBP-1 activity in urine and serum was used for evaluation of the degree of graft ischemia during human kidney transplantation. The aim of our present research was to determine FBP-1 activity in urine as an indicator of damage to renal proximal tubules in children with idiopathic nephrotic syndrome (INS).

Methods: We evaluated the excretion of FBP-1 into urine of 21 children (10 girls and 11 boys) with INS, aged from 10 to 15 years and 30 healthy children (14 girls and 16 boys), aged from 2 to 15 years. FBP-1 activity was determined by the Latzko and Gibbs method. Creatinine (mg%) in urine and blood serum was measured by the Jaffe method in Larsen modification. Protein in blood serum was determined by the biuret method (g/L), and albumin (mg%) by the Young method. Proteinuria in the urine collected over 24 h was measured with the Exton turbidimetric method by Tomaszewski with modification and expressed in mg/kg body weight/24 h.

Results: In the urine of 30 healthy children, FBP-1 activity was in the range from 0–1.74 μmol FPB/h/mmol of creatinine. In 43% of the healthy children, FBP-1 activity in urine was not detectable. In the period of intensive proteinuria during the INS in children, FBP-1 activity and protein concentrations in urine were significantly higher than in the control group (p<0.0008 and p<0.0001, respectively). In the urine of children with active INS, we observed a very weak negative linear correlation between protein concentration and FBP-1 activity (r=−0.5018, p=0.067). After treatment with Encorton (prednisone), FBP-1 activity and protein concentration in urine dropped to values of the control group.

Conclusions: “The overload” of proximal renal tubules by proteins in children with INS releases FBP-1 into urine. FBP-1 activity in urine may therefore be considered as a marker of damage to the proximal renal tubules in children with INS.

Clin Chem Lab Med 2008;46:831–5.

Keywords: fructose-1,6-bisphosphatase (FBP-1); idiopathic nephrotic syndrome; renal proximal tubules

About the article

Corresponding author: Sławomir D. Szajda, PhD, Assistant Professor, Department of Pharmaceutical Biochemistry, Medical University, Mickiewicza Str. 2A, 15-230 Bialystok, Poland Phone: +48-85-748-5690/748-5691,


Received: 2007-01-01

Accepted: 2008-02-02

Published in Print: 2008-06-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 46, Issue 6, Pages 831–835, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.171.

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