Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 46, Issue 6

Issues

Polymorphisms of CYP17A1, CYP19, and androgen in Brazilian women with uterine leiomyomas

Fabíola Encinas Rosa
  • 1Department of Genetics, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Renata de Azevedo Canevari
  • 2Department of Urology, Faculty of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eliane Papa Ambrosio
  • 3Department of Genetics, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Priscila Daniele Ramos Cirilo
  • 4Department of Genetics, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anaglória Pontes
  • 5Department of Obstetrics and Gynecology, Faculty of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cláudia Aparecida Rainho
  • 6Department of Genetics, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Silvia Regina Rogatto
  • 7Department of Urology, Faculty of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Background: Uterine leiomyomas are common, benign, smooth muscle tumors representing a significant public health problem. The aim of this study was to investigate CYP17A1, CYP19, and androgen (AR) polymorphisms, their relative risks for uterine leiomyomas and possible associations with clinical parameters.

Methods: Uterine leiomyoma tissues and blood samples were obtained from 87 patients, as were peripheral blood samples from 68 control women. Clinical data were recorded in both groups. The CYP17A1 (rs743572) polymorphism was analyzed by PCR-RFLP, and the CYP19 [TTTA]n repeat and AR [CAG]n repeat were analyzed using PCR-based GeneScan analysis. AR loss of heterozygosity (LOH) and microsatellite instability were also evaluated, while samples exhibiting LOH were analyzed for X inactivation.

Results: Clinical parameters related to disease development did not differ between cases and controls. CYP17A1 *A2/*A2 genotype was prevalent in non-white women. CYP17A1, CYP19, and AR genotypes and alleles did not differ between groups. However, alleles presenting [TTTA]7 repeats in intron 4 of CYP19 were more frequent in the control group (p=0.0550). Shorter and longer [CAG]n repeat alleles of AR were exclusive to the leiomyoma group. The LOH assay showed allele losses at AR locus in four informative tumors and X chromosome inactivation analysis revealed that these tumors retained the active allele.

Conclusions: The overall lack of association between uterine leiomyomas with polymorphisms involved in steroidogenesis or steroid metabolism is consistent with the hypothesis that these polymorphisms do not substantially contribute to the development of these tumors.

Clin Chem Lab Med 2008;46:814–23.

Keywords: androgen (AR); CYP17A1; CYP19; polymorphism; uterine leiomyoma

About the article

Corresponding author: Silvia Regina Rogatto, PhD, NeoGene Laboratory, Departamento de Urologia, Faculdade de Medicina, UNESP, Botucatu, 18618-000, São Paulo, Brazil Phone: +55-14-3811-6436, Fax: +55-14-3811-6271,


Received: 2007-10-05

Accepted: 2008-02-26

Published in Print: 2008-06-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 46, Issue 6, Pages 814–823, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.172.

Export Citation

©2008 by Walter de Gruyter Berlin New York.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Noor Ahmad Shaik, Sujatha Govindan, Vijayalakshmi Kodati, Kaipa Prabhakar Rao, and Qurratulain Hasan
Hematology/Oncology and Stem Cell Therapy, 2009, Volume 2, Number 1, Page 289
[2]
Angela T. Alleyne, Shane Austin, and Angela Williams
Meta Gene, 2014, Volume 2, Page 358

Comments (0)

Please log in or register to comment.
Log in