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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 46, Issue 8

Issues

Can both EDTA and citrate plasma samples be used in measurements of fibrinogen and C-reactive protein concentrations?

Mika Skeppholm
  • 1Department of Clinical Sciences, Karolinska Institutet, Danderyd Hospital, Division of Cardiology, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ N. Håkan Wallén
  • 2Department of Clinical Sciences, Karolinska Institutet, Danderyd Hospital, Division of Cardiology, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Margareta Blombäck
  • 3Department of Molecular Medicine and Surgery, Karolinska Institutet, Division of Clinical Chemistry and Blood Coagulation Research, Karolinska University Hospital, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anders Kallner
  • 4Department of Molecular Medicine and Surgery, Karolinska Institutet, Division of Clinical Chemistry and Blood Coagulation Research, Karolinska University Hospital, Stockholm, Sweden
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-06-20 | DOI: https://doi.org/10.1515/CCLM.2008.219

Abstract

Background: Fibrinogen and C-reactive protein (CRP) concentrations are predictors of outcome in the atherosclerotic patient. It is important in risk stratification that these quantities are measured reproducibly in routine and research.

Method: In the present study, we compare measurements of fibrinogen and high-sensitivity CRP in EDTA and citrate plasma samples (n=150) using nephelometric immunoassays. Fibrinogen was also measured in citrate plasma using a clotting method.

Results: In approximately one-third of the samples, the fibrinogen concentration measured by immunoassay was higher in citrate plasma than in EDTA plasma, in spite of the dilution by citrate. The immunoassay results of fibrinogen concentration measurements in EDTA and citrate plasma differed significantly and also differed from those of functionally measured fibrinogen concentrations. A difference was found between the concentration of CRP in EDTA plasma and citrated plasma which also did not correspond to the dilution.

Conclusions: Reproducibility of results is essential in risk stratification by fibrinogen or high-sensitivity CRP concentrations and small differences close to the decision limits may have a decisive impact. Immunological measurements are liable to confounding effects that may be difficult to foresee, qualitatively and quantitatively. Great care should be observed when measuring the concentration of calcium containing analytes in anticoagulated samples. Fibrinogen concentrations should preferably be measured functionally in citrate plasma.

Clin Chem Lab Med 2008;46:1175–9.

Keywords: citrate; C-reactive protein; EDTA; fibrinogen

About the article

Corresponding author: Mika Skeppholm, Department of Clinical Sciences, Karolinska Institutet, Danderyd Hospital, Division of Cardiology, Stockholm, Sweden Phone: +46-8-6555000,


Received: 2007-11-05

Accepted: 2008-04-10

Published Online: 2008-06-20

Published in Print: 2008-08-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 46, Issue 8, Pages 1175–1179, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.219.

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