Clinical Chemistry and Laboratory Medicine (CCLM)
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Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
1Institute of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
2Diagnostics Research Department, Pliva-Lachema Diagnostika, Brno, Czech Republic
3Department of Clinical Biochemistry, Municipal Hospital, Čáslav, Czech Republic
Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 46, Issue 8, Pages 1127–1133, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2008.224, August 2008
Background: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods.
Method: Spectrophotometry was used.
Results: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards.
Conclusions: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.
Clin Chem Lab Med 2008;46:1127–33.
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