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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 46, Issue 9

Issues

Diagnostic performance of rapid tests for detection of fecal calprotectin and lactoferrin and their ability to discriminate inflammatory from irritable bowel syndrome

Charlotte M.T. Otten / Liselotte Kok
  • 2Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ben J.M. Witteman
  • 3The Department of Gastroenterology and Hepatology, Gelderse Vallei Hospital, Ede, The Netherlands
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  • De Gruyter OnlineGoogle Scholar
/ Ruben Baumgarten
  • 4Department of Clinical Biochemistry and Hematology, Gelderse Vallei Hospital, Ede, The Netherlands
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/ Ellen Kampman / Karel G.M. Moons
  • 6Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands
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/ Niek J. de Wit
  • 7Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-07-02 | DOI: https://doi.org/10.1515/CCLM.2008.246

Abstract

Background: Ruling out somatic bowel disease, such as inflammatory bowel disease (IBD), is an important goal in the management of abdominal complaints. Endoscopy is commonly used but is invasive and expensive. Mucosal inflammation in IBD can be detected through fecal biomarkers, though the present enzyme-linked immunoabsorbent assay (ELISA) tests require laboratory facilities. We validated the diagnostic performance of two new fecal rapid tests (FRTs) for the detection of calprotectin and lactoferrin and assessed their potential to differentiate IBD from irritable bowel syndrome (IBS).

Methods: The calprotectin and lactoferrin FRTs and ELISA tests were performed on the fecal samples of 114 patients referred for endoscopy, 80% of whom had IBS and 20% IBD, and validated against the endoscopic diagnosis.

Results: The sensitivity and negative predictive value of the calprotectin FRT were both 100%, whereas they were 78% and 95%, respectively, for the lactoferrin FRT. The specificity and positive predictive value were slightly higher for the lactoferrin FRT. Both FRTs had similar diagnostic accuracy as the corresponding ELISA tests.

Conclusions: The calprotectin and lactoferrin rapid tests are as good as the ELISA tests in detecting colonic inflammation. Given their simple use, FRTs can support the non-invasive exclusion of IBD, notably in primary care.

Clin Chem Lab Med 2008;46:1275–80.

Keywords: biomarkers; enzyme-linked immunosorbent assay (ELISA); fecal calprotectin; fecal lactoferrin; inflammatory bowel disease; irritable bowel syndrome

About the article

Corresponding author: Dr. N.J. de Wit, Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands Phone: +31-88-5768247,


Received: 2008-02-14

Accepted: 2008-04-28

Published Online: 2008-07-02

Published in Print: 2008-09-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 46, Issue 9, Pages 1275–1280, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2008.246.

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©2008 by Walter de Gruyter Berlin New York.Get Permission

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