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Clinical Chemistry and Laboratory Medicine (CCLM)

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Volume 47, Issue 1 (Jan 2009)


Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry

Lamia Benezzeddine-Boussaidi
  • 1Centre National de Médecine et des Sciences du Sport, Unité de recherche Evaluation Sport Santé, Tunis, Tunisia
/ Georges Cazorla
  • 2Université Victor Segalen Bordeaux 2, Faculté des Sciences du Sport et de l'Education Physique, Laboratoire Evaluation Sport Santé, Bordeaux, France
/ Anne-Marie Melin
  • 3Université Victor Segalen Bordeaux 2, INSERM U577, Bordeaux, France
Published Online: 2008-12-31 | DOI: https://doi.org/10.1515/CCLM.2009.007


Background: The objective of this study was to develop a robust quantification method for simultaneously analyzing molecules in human plasma using the Fourier transform infrared (FT-IR) system with a partial least square (PLS) regression.

Methods: Plasma spectra were analyzed from 4000 to 500 cm−1 (with 2.0 cm−1 of resolution and 32 scans), and the molecule concentrations (IgA, IgG, IgM) were measured blindly by using a cross-validation model prepared by PLS analysis of data from 135 samples.

Results: There was a significant correlation between the FT-IR predicted concentration and the concentration obtained with the clinical reference method: R2=0.98 (IgA), R2=0.98 (IgG), and R2=0.97 (IgM). The root mean square error of prediction (RMSEP) was 0.05 g·L−1 (IgA), 0.4 g·L−1 (IgG), and 0.03 g·L−1 (IgM). Variability of inter-experimenter reproducibility was less than 2%. The interchangeability of the two methods was studied by using the Bland-Altman method.

Conclusions: Together with PLS analysis, FT-IR spectrometry appears to be an easy-to-use and accurate method to determine multianalyte concentrations in dried human plasma. It could be an alternative tool for rapidly quantifying many molecules after developing a specific predictive model.

Clin Chem Lab Med 2009;47:83–90.

Keywords: immunoglobulins; multivariate analysis; plasma molecules; Fourier transform infrared (FT-IR) spectrometry; validation

About the article

Corresponding author: Georges Cazorla, Université Victor Segalen Bordeaux 2, Faculté des Sciences du Sport et de l'Éducation Physique, Laboratoire Evaluation Sport Santé, 12 Avenue Camille Julian, 33607 Pessac, France Phone: +33-5-56845227, Fax: +33-5-56845235,

Received: 2008-04-30

Accepted: 2008-09-01

Published Online: 2008-12-31

Published in Print: 2009-01-01

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.007. Export Citation

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