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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

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1437-4331
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Volume 47, Issue 1 (Jan 2009)

Issues

Aging and endothelial progenitor cell telomere length in healthy men

Erich J. Kushner
  • 1Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gary P. Van Guilder
  • 2Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Owen J. MacEneaney
  • 3Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jennifer N. Cech
  • 4Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Brian L. Stauffer
  • 5Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA, Department of Medicine, University of Colorado at Denver, Denver, CO, USA and Denver Health Medical Center, Denver, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Christopher A. DeSouza
  • 6Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA and Department of Medicine, University of Colorado at Denver, Denver, CO, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-12-05 | DOI: https://doi.org/10.1515/CCLM.2009.016

Abstract

Background: Telomere length declines with age in mature endothelial cells and is thought to contribute to endothelial dysfunction and atherogenesis. Bone marrow-derived circulating endothelial progenitor cells (EPCs) are critical to vascular health as they contribute to both reendothelialization and neovascularization. We tested the hypothesis that EPC telomere length decreases with age in healthy adult humans.

Methods: Peripheral blood samples were collected from 40 healthy, non-obese, sedentary men: 12 young (age 21–34 years), 12 middle-aged (43–55 years) and 16 older (57–68 years). Putative EPCs were isolated from peripheral blood mononuclear cells and telomere length was determined using genomic DNA preparation and Southern hybridization techniques.

Results: EPC telomere length (base pairs) was ∼20% (p=0.01) lower in the older (8492+523 bp) compared to the middle-aged (10,565+572 bp) and young (10,205+501 bp) men. Of note, there was no difference in EPC telomere length between the middle-aged and young men.

Conclusions: These results demonstrate that EPC telomere length declines with age in healthy, sedentary men. Interestingly, telomere length was well preserved in the middle-aged compared to young men, suggesting that EPC telomere shortening occurs after the age of 55 years.

Clin Chem Lab Med 2009;47:47–50.

Keywords: aging; endothelial progenitor cells; telomeres

About the article

Corresponding author: Christopher DeSouza, PhD, Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, 354 UCB, Boulder, CO 80309, USA Phone: +1-303-492-2988, Fax: +1-303-492-6778,


Received: 2008-07-07

Accepted: 2008-08-26

Published Online: 2008-12-05

Published Online: 2008-12-05

Published in Print: 2009-01-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.016.

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