Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

249,00 € / $374.00 / £187.00*

Online
ISSN
1437-4331
See all formats and pricing

 


Select Volume and Issue

Issues

30,00 € / $42.00 / £23.00

Get Access to Full Text

Aging and endothelial progenitor cell telomere length in healthy men

Erich J. Kushner1 / Gary P. Van Guilder2 / Owen J. MacEneaney3 / Jennifer N. Cech4 / Brian L. Stauffer5 / Christopher A. DeSouza6

1Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA

2Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA

3Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA

4Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA

5Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA, Department of Medicine, University of Colorado at Denver, Denver, CO, USA and Denver Health Medical Center, Denver, CO, USA

6Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, Boulder, CO, USA and Department of Medicine, University of Colorado at Denver, Denver, CO, USA

Corresponding author: Christopher DeSouza, PhD, Department of Integrative Physiology, Integrative Vascular Biology Laboratory, University of Colorado, 354 UCB, Boulder, CO 80309, USA Phone: +1-303-492-2988, Fax: +1-303-492-6778,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 47, Issue 1, Pages 47–50, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2009.016, December 2008

Publication History

Received:
2008-07-07
Accepted:
2008-08-26
Published Online:
2008-12-05

Abstract

Background: Telomere length declines with age in mature endothelial cells and is thought to contribute to endothelial dysfunction and atherogenesis. Bone marrow-derived circulating endothelial progenitor cells (EPCs) are critical to vascular health as they contribute to both reendothelialization and neovascularization. We tested the hypothesis that EPC telomere length decreases with age in healthy adult humans.

Methods: Peripheral blood samples were collected from 40 healthy, non-obese, sedentary men: 12 young (age 21–34 years), 12 middle-aged (43–55 years) and 16 older (57–68 years). Putative EPCs were isolated from peripheral blood mononuclear cells and telomere length was determined using genomic DNA preparation and Southern hybridization techniques.

Results: EPC telomere length (base pairs) was ∼20% (p=0.01) lower in the older (8492+523 bp) compared to the middle-aged (10,565+572 bp) and young (10,205+501 bp) men. Of note, there was no difference in EPC telomere length between the middle-aged and young men.

Conclusions: These results demonstrate that EPC telomere length declines with age in healthy, sedentary men. Interestingly, telomere length was well preserved in the middle-aged compared to young men, suggesting that EPC telomere shortening occurs after the age of 55 years.

Clin Chem Lab Med 2009;47:47–50.

Keywords: aging; endothelial progenitor cells; telomeres

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Mark D. Ross, Eva Malone, and Geraint Florida-James
Oxidative Medicine and Cellular Longevity, 2016, Volume 2016, Page 1
[2]
Yubo Tang, Angela Jacobi, Corina Vater, Lijin Zou, Xuenong Zou, and Maik Stiehler
STEM CELLS, 2015, Volume 33, Number 6, Page 1863
[3]
Yubo Tang, Corina Vater, Angela Jacobi, Cornelia Liebers, Xuenong Zou, and Maik Stiehler
British Journal of Pharmacology, 2014, Volume 171, Number 9, Page 2440
[4]
O. S. Al-Attas, N. M. Al-Daghri, M. S. Alokail, A. Alfadda, A. Bamakhramah, S. Sabico, D. Pritlove, A. Harte, G. Tripathi, P. G. McTernan, S. Kumar, and G. Chrousos
European Journal of Endocrinology, 2010, Volume 163, Number 4, Page 601
[5]
Noboru Toda
Pharmacology & Therapeutics, 2012, Volume 133, Number 2, Page 159
[6]
A. Dei Cas, V. Spigoni, L. Franzini, M. Preti, D. Ardigò, E. Derlindati, M. Metra, L.D. Monti, P. Dell’Era, L. Gnudi, and I. Zavaroni
Nutrition, Metabolism and Cardiovascular Diseases, 2013, Volume 23, Number 3, Page 272
[7]
Erich J. Kushner, Owen J. MacEneaney, Brian R. Weil, Jared J. Greiner, Brian L. Stauffer, and Christopher A. DeSouza
Journal of Vascular Research, 2011, Volume 48, Number 5, Page 408
[8]
Bert R. Everaert, Emeline M. Van Craenenbroeck, Vicky Y. Hoymans, Steven E. Haine, Luc Van Nassauw, Viviane M. Conraads, Jean-Pierre Timmermans, and Christiaan J. Vrints
International Journal of Cardiology, 2010, Volume 144, Number 3, Page 350
[9]
Owen J. MacEneaney, Erich J. Kushner, Christian M. Westby, Jennifer N. Cech, Jared J. Greiner, Brian L. Stauffer, and Christopher A. DeSouza
Obesity, 2010, Volume 18, Number 9, Page 1677
[10]
E. Kushner, G. Van Guilder, O. MacEneaney, J. Greiner, J. Cech, B. Stauffer, and C. DeSouza
Age and Ageing, 2010, Volume 39, Number 2, Page 268

Comments (0)

Please log in or register to comment.