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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 47, Issue 11

Issues

Serum albumin fragmentation in end-stage renal disease patients – a pilot study

Elena Donadio
  • Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Francesco Piccolomini / Veronica Dimuccio / Antonio Felicioli
  • Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ettore Balestreri / Riccardo Cianti / Alessandro Armini / Luca Bini / Romano Felicioli
  • Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Carlo Donadio
Published Online: 2009-09-25 | DOI: https://doi.org/10.1515/CCLM.2009.303

Abstract

Background: The goal of this study was to detect modification in the expression of plasma proteins and/or post-translational modifications of their structure in patients with end stage renal disease.

Methods: Serum samples from 19 adult patients treated by maintenance hemodialysis (MHD) were analyzed in comparison to sera from six healthy controls using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2DE). Spots of interest were identified by mass spectrometry analysis. In addition, the 2DE maps were incubated with a human anti-albumin polyclonal antibody.

Results: SDS-PAGE gels, 2DE maps and matrix-assisted laser desorption/ionization time of flight analysis indicated over-expression of low-molecular weight proteins (LMWP) in sera from patients. Unexpectedly, another 15 spots with estimated Mr of 12.5–29 kDa from the 2DE maps of six patients were identified as fragments of albumin. 2D immunoblotting of sera from 12 other patients detected numerous albumin fragments.

Conclusions: These results indicate that in addition to increased expression of LMWP, a relevant amount of albumin fragments are detectable in the serum of patients undergoing MHD. Uremia appears to facilitate the fragmentation of albumin and/or the retention of albumin fragments in blood.

Clin Chem Lab Med 2009;47:1373–9.

Keywords: albumin fragments; immunoblotting; low-molecular weight proteins; matrix-assisted laser desorption/ionization time of flight mass spectrometry; plasma proteomics; uremic syndrome

About the article

Corresponding author: Prof. Carlo Donadio, Dipartimento di Medicina Interna, Nefrologia, Via Roma 67, 56100 Pisa, Italy Phone: +39 050 553414, Fax: +39 050 997278,


Received: 2009-05-19

Accepted: 2009-07-26

Published Online: 2009-09-25

Published in Print: 2009-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 11, Pages 1373–1379, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.303.

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