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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 47, Issue 11

Issues

Detection of myelin autoantibodies: evaluation of an assay system for diagnosis of multiple sclerosis in differentiation from other central nervous system diseases

Markus Langkamp
  • Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Reutlingen, Germany
  • Other articles by this author:
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/ Stephanie C. Hörnig
  • Gesellschaft zur Förderung von Medizin-, Bio-, und Umwelttechnologien e.V., Fachsektion Halle Halle (Saale), Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Joachim B. Hörnig
  • Gesellschaft zur Förderung von Medizin-, Bio-, und Umwelttechnologien e.V., Fachsektion Halle Halle (Saale), Germany
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  • De Gruyter OnlineGoogle Scholar
/ Marion Kirschner
  • Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Reutlingen, Germany
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/ Lutz Pridzun
  • Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Reutlingen, Germany
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/ Malte E. Kornhuber
  • Martin-Luther Universität Halle-Wittenberg, Universitätsklinik und Poliklinik für Neurologie, Halle (Saale), Germany
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Abstract

Background: Multiple sclerosis (MS) is a frequent and often severe autoimmune disease of the central nervous system. We describe a newly developed enzyme-linked immunosorbent assay (ELISA)-based test system for the assessment of neuronal autoantibodies in serum and cerebrospinal fluid (CSF). This tool could help define autoimmune status and thus be a potential means of therapeutic surveillance.

Methods: We used an assay system (ELISA, E100, Mediagnost) based on purified bovine antigens [myelin-oligodendrocyte glycoprotein (MOG), myelin basic protein (MBP), myelin-associated glycoprotein (MAG), proteolipid protein (PLP) and alpha-B-crystalline (CRY)] antibodies for the measurement of specific immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies. Assay characteristics and preliminary validation were conducted by measurement of serum and CSF samples from 41 MS patients and 128 patients with other neurological diseases (OND).

Results: We measured the inter- (17.8/10.1%) and intra-assay variability (5.5/6.7%); linearity (1:250– 1:16,000), and specificity of IgG and IgM. We demonstrate that by the results of this test system MS patients can be differentiated from patients with OND.

Conclusions: The ELISA kit we evaluated is suitable for the measurement of neuronal autoantibodies. The initial validation demonstrates its potential use in the differential diagnosis of central neuronal system diseases.

Clin Chem Lab Med 2009;47:1395–400.

Keywords: autoantibody; ELISA; multiple sclerosis; myelin antigen; neuronal antigen

About the article

Corresponding author: Markus Langkamp, Mediagnost GmbH, Aspenhaustr. 25, 72770 Reutlingen, Germany Phone: +49 7121 51 484 0, Fax: +49 7121 51 484 10,


Received: 2009-05-28

Accepted: 2009-07-28

Published in Print: 2009-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 11, Pages 1395–1400, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.313.

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