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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
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1437-4331
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Volume 47, Issue 11

Issues

Potential of the Sebia Capillarys® neonat fast automated system for neonatal screening of sickle cell disease

Gilles Renom
  • Laboratoire de Dépistage Périnatal, Centre de Biologie et Pathologie, CHRU de Lille, Lille, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Claude Mereau
  • Laboratoire de Diagnostic des Hémoglobinopathies, Centre de Biologie et Pathologie, CHRU de Lille, Lille, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Patrice Maboudou
  • Laboratoire de Diagnostic des Hémoglobinopathies, Centre de Biologie et Pathologie, CHRU de Lille, Lille, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jean-Marc Périni
  • Laboratoire de Dépistage Périnatal, Centre de Biologie et Pathologie, CHRU de Lille, Lille, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Background: Most screening programs for sickle cell disease (SCD) utilize isoelectric focusing (IEF) or high performance liquid chromatography (HPLC) to detect haemoglobin (Hb) variants. The first method is not automated and becomes too tedious when many samples have to be investigated. The aim of this work is to explore the capacity of an automated capillary electrophoresis (CE) system, with full traceability, as a tool for newborn screening of SCD.

Methods: The Capillarys® neonat fast automated system has been developed by Sebia for newborn screening. We performed separate studies using different types of samples to evaluate the utility of the Capillarys® for (i) separating Hb S and other variants, and (ii) for performing the routine activity of our laboratory for 20 working days.

Results: A throughput of 48 samples per hour with a loading capacity of 192 samples was achieved. Migration times of the major Hb variants were distinct. There were few variants showing similar migration times to Hb S and Hb C and thalassaemia could be detected. In addition, late screening, screening of premature or transfused babies and screening performed using poor quality Guthrie's cards did not interfere with reporting of accurate phenotypes.

Conclusions: Sebia Capillarys® neonat fast automated system is a reliable tool for haemoglobinopathy neonatal screening.

Clin Chem Lab Med 2009;47:1423–32.

Keywords: capillary electrophoresis; newborn screening; sickle cell disease; thalassaemia

About the article

Corresponding author: Jean-Marc Périni, Laboratoire de Dépistage Périnatal, Centre de Biologie et Pathologie, CHRU de Lille, 59037 Lille Cedex, France Phone: +33 3 20 44 49 87, Fax: +33 3 20 44 49 57,


Received: 2009-05-12

Accepted: 2009-07-30

Published in Print: 2009-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 11, Pages 1423–1432, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.315.

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