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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

Online
ISSN
1437-4331
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Volume 47, Issue 2

Issues

Methodology for monitoring cytomegalovirus infection after renal transplantation

Wujun Xue
  • 1Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Hua Liu
  • 2Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Hang Yan
  • 3Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Puxun Tian
  • 4Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Xiaoming Ding
  • 5Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Xiaoming Pan
  • 6Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Xinshun Feng
  • 7Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Heli Xiang
  • 8Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Jun Hou
  • 9Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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/ Xiaoli He
  • 10Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China
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Abstract

Background: The aim of this study was to evaluate the diagnostic value of different detection methods for cytomegalovirus (CMV) infection after renal transplantation and also to establish a system to monitor therapy for CMV infection.

Methods: We retrospectively studied 1516 renal transplant recipients from June 1994 to December 2006. All patients were screened for CMV-DNA. A total of 1402 patients had received CMV-IgG/IgM detection since June 1996 and 660 had received CMV antigen detection since June 2000.

Results: A total of 664 (43.8%) recipients developed CMV infection. The sensitivity, specificity and Youden index of the three methods, respectively, were 18.84%, 100% and 0.1884 for ELISA, 91.86%, 82.98% and 0.7484 for PCR, and 88.06%, 96.95% and 0.8501 for the CMV-pp65 antigenemia test. The sensitivity and specificity of the two combined detection methods (CMV-DNA and CMV-pp65) for post-operation CMV infection were 93.49% and 99.06%; the two detection methods had significant dependability (p<0.05) in diagnosis of CMV infection and in evaluation of therapeutic effect of antiviral drugs.

Conclusions: Only ELISA can be used as a screening index in order to distinguish whether the donors or recipients are infected with CMV or not. CMV-pp65 antigenemia can help guide clinical therapy for CMV infection. CMV-pp65 and CMV-PCR combined together provide a more effective method to monitor CMV infection and predict its outcome.

Clin Chem Lab Med 2009;47:177–81.

Keywords: cytomegalovirus; diagnosis; infection; methodology; renal transplantation

About the article

Corresponding author: Wujun Xue, Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China Phone: +86-29-85323955, Fax: +86-29-85323718,


Received: 2008-07-12

Accepted: 2008-11-06

Published in Print: 2009-02-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 2, Pages 177–181, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.033.

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