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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 47, Issue 4

Issues

Translation termination and protein folding pathway genes are not correlated in gastric cancer

Joana Malta-Vacas / Sofia Nolasco / Carolino Monteiro / Helena Soares
  • Escola Superior de Tecnologia da Saúde de Lisboa, Lisboa, Portugal
  • Instituto Gulbenkian de Ciência, Oeiras, Portugal
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Miguel Brito
Published Online: 2009-03-02 | DOI: https://doi.org/10.1515/CCLM.2009.091

Abstract

Background: The eukaryotic release factor 3 (eRF3) has been shown to affect both tubulin and actin cytoskeleton, suggesting a role in cytoskeleton assembly, mitotic spindle formation and chromosome segregation. Also, direct interactions between eRF3 and subunits of the cytosolic chaperonin CCT have been described. Moreover, both eRF3a and CCT subunits have been described to be up-regulated in cancer tissues. Our aim was to evaluate the hypothesis that eRF3 expression levels are correlated with the expression of genes encoding proteins involved in the tubulin folding pathways.

Methods: Relative expression levels of eRF1, eRF3a/GSPT1, PFDN4, CCT2, CCT4, and TBCA genes in tumour samples relative to their adjacent normal tissues were investigated using real time-polymerase chain reaction in 20 gastric cancer patients.

Results: The expression levels of eRF3a/GSPT1 were not correlated with the expression levels of the other genes studied. However, significant correlations were detected between the other genes, both within intestinal and diffuse type tumours.

Conclusions: eRF3a/GSPT1 expression at the mRNA level is independent from both cell translation rates and from the expression of the genes involved in tubulin-folding pathways. The differences in the patterns of expression of the genes studied support the hypothesis of genetically independent pathways in the origin of intestinal and diffuse type gastric tumours.

Clin Chem Lab Med 2009;47:427–31.

Keywords: CCT subunits; eukaryotic release factor 3a/G-to-S phase transition 1 (eRF3a/GSPT1); gastric cancer; protein folding pathways; translation termination

About the article

Corresponding author: Prof. Doutor Miguel Brito, Escola Superior de Tecnologia da Saúde de Lisboa, Av. D. João II lote 4.69.01, 1990-096 Lisboa, Portugal Phone: +351-218980400, Fax: +351-218980460,


Received: 2008-09-05

Accepted: 2009-01-05

Published Online: 2009-03-02

Published in Print: 2009-04-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 4, Pages 427–431, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.091.

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[1]
Joana Malta-Vacas, Paula Ferreira, Carolino Monteiro, and Miguel Brito
Cancer Genetics and Cytogenetics, 2009, Volume 195, Number 2, Page 132

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