Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 47, Issue 4


Novel serum paraoxonase activity assays are associated with coronary artery disease

Nicola Martinelli / Domenico Girelli / Oliviero Olivieri / Patrizia Guarini / Antonella Bassi / Elisabetta Trabetti
  • Section of Biology and Genetics, Department of Mother and Child and Biology – Genetics, University of Verona, Verona, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Simonetta Friso / Francesca Pizzolo / Claudia Bozzini / Ilaria Tenuti / Laura Annarumma / Renzo Schiavon / Pier Franco Pignatti
  • Section of Biology and Genetics, Department of Mother and Child and Biology – Genetics, University of Verona, Verona, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Roberto Corrocher
Published Online: 2009-03-11 | DOI: https://doi.org/10.1515/CCLM.2009.108


Background: Serum paraoxonase (PON1) exerts antiatherogenic effects. Novel PON1 enzymatic tests have been recently developed: 5-thiobutyl butyrolactone (TBBL) estimates PON1 lactonase activity, whereas 7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin (DEPCyMC) is considered a surrogate marker of PON1 concentration. The TBBL to DEPCyMC ratio provides the normalized lactonase activity (NLA), which may reflect the degree of PON1 lactonase catalytic stimulation. The aim of this study was to evaluate for the first time TBBLase and DEPCyMCase activity in patients with coronary artery disease (CAD).

Methods: An angiography-based case-control study was conducted, including 300 sex- and age-matched subjects [100 CAD-free, 100 CAD without myocardial infarction (MI) and 100 CAD with MI].

Results: A low DEPCyMCase activity (lowest vs. highest tertile: OR 2.96, 95% CI 1.18–7.43) and a high NLA (highest vs. lowest tertile: OR 3.25, 95% CI 1.28–8.26) were both associated with CAD, independent of classical atherosclerosis risk factors, lipid-lowering therapy and PON1 genotype. Total TBBLase activity was, however, not different in CAD compared to CAD-free subjects.

Conclusions: Novel PON1 activity assays may be associated with CAD. In this study, CAD patients had low DEPCyMCase activity, a possible marker of low PON1 concentration, but showed a high stimulation of PON1 lactonase activity.

Clin Chem Lab Med 2009;47:432–40.

Keywords: coronary artery disease; DEPCyMCase activity; serum paraoxonase; TBBLase activity

About the article

Corresponding author: Nicola Martinelli, MD, Department of Clinical and Experimental Medicine, University of Verona, Policlinico G.B. Rossi, 37134 Verona, Italy Phone: +39-045-8074401, Fax: +39-045-8027473,

Received: 2008-10-08

Accepted: 2009-01-17

Published Online: 2009-03-11

Published in Print: 2009-04-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 4, Pages 432–440, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.108.

Export Citation

©2009 by Walter de Gruyter Berlin New York.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

J. Allen Crow, Edward C. Meek, Robert W. Wills, and Janice E. Chambers
Diabetes/Metabolism Research and Reviews, 2017, Page e2967
Joussef Hayek, Carlo Cervellati, Ilaria Crivellari, Alessandra Pecorelli, and Giuseppe Valacchi
Oxidative Medicine and Cellular Longevity, 2017, Volume 2017, Page 1
Carlo Cervellati, Gloria Bonaccorsi, Alessandro Trentini, Giuseppe Valacchi, Juana M. Sanz, Monica Squerzanti, Manuela Spagnolo, Leo Massari, Ilaria Crivellari, Pantaleo Greco, Roberta Parladori, Angelina Passaro, and Giorgio Ricci
Scandinavian Journal of Clinical and Laboratory Investigation, 2017, Page 1
Nicola Martinelli, Anabel García-Heredia, Helena Roca, Núria Aranda, Victoria Arija, Bharti Mackness, Michael Mackness, Fabiana Busti, Gerard Aragonès, Juan Pedro-Botet, Federica Pedica, Ivana Cataldo, Judit Marsillach, Jorge Joven, Domenico Girelli, and Jordi Camps
Journal of Lipid Research, 2013, Volume 54, Number 5, Page 1484
Carlo Cervellati, Alessandro Trentini, Arianna Romani, Tiziana Bellini, Cristina Bosi, Beatrice Ortolani, Amedeo Zurlo, Angelina Passaro, Davide Seripa, and Giovanni Zuliani
Journal of Neurochemistry, 2015, Volume 135, Number 2, Page 395
Y. Zhang, H. Liu, J. He, K. Xu, H. Bai, Y. Wang, F. Zhang, J. Zhang, L. Cheng, and P. Fan
European Journal of Endocrinology, 2015, Volume 172, Number 4, Page 391
Michael Aviram and Jacob Vaya
Current Opinion in Lipidology, 2013, Volume 24, Number 4, Page 339
Muiruri Macharia, Mogamat S. Hassan, Diane Blackhurst, Rajiv T. Erasmus, and Tandi E. Matsha
Journal of Cardiovascular Medicine, 2012, Volume 13, Number 7, Page 443
Alejandro Gugliucci, Kazuhiko Kotani, and Satoshi Kimura
Journal of Lipids, 2012, Volume 2012, Page 1
Mingsong Wang, Xilong Lang, Shitao Cui, Liangjian Zou, Jia Cao, Sheng Wang, and Xintian Wu
DNA and Cell Biology, 2012, Volume 31, Number 6, Page 975
Yuan Zhao, Yushui Ma, Ying Fang, Lili Liu, Shengdi Wu, Da Fu, and Xiaofeng Wang
Molecular Genetics and Metabolism, 2012, Volume 105, Number 1, Page 141
Carlos Alonso-Villaverde Lozano
Enfermedades Infecciosas y Microbiología Clínica, 2009, Volume 27, Page 33
Nicola Martinelli, Roberta Micaglio, Letizia Consoli, Patrizia Guarini, Elisa Grison, Francesca Pizzolo, Simonetta Friso, Elisabetta Trabetti, Pier Franco Pignatti, Roberto Corrocher, Oliviero Olivieri, and Domenico Girelli
Experimental Diabetes Research, 2012, Volume 2012, Page 1
Hagai Tavori, Michael Aviram, Soliman Khatib, Ramadan Musa, Dalit Mannheim, Ron Karmeli, and Jacob Vaya
Free Radical Biology and Medicine, 2011, Volume 50, Number 1, Page 148
Jordi Camps, Michael Mackness, Bharti Mackness, Judit Marsillach, and Jorge Joven
Clinical Chemistry and Laboratory Medicine, 2010, Volume 48, Number 6
Mariann Harangi, Ildikó Seres, János Harangi, and György Paragh
Cardiovascular Drugs and Therapy, 2009, Volume 23, Number 6, Page 501

Comments (0)

Please log in or register to comment.
Log in