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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
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Volume 47, Issue 5 (May 2009)

Issues

A routine method for the quantification of the novel antimycotic drug posaconazole in plasma using liquid chromatography-tandem mass spectrometry

Michael Vogeser / Christina Rieger / Helmut Ostermann / Ute Spöhrer
Published Online: 2009-03-23 | DOI: https://doi.org/10.1515/CCLM.2009.129

Abstract

Background: Posaconazole is now widely used for prophylaxis of invasive fungal infections in immunocompromised patients. The pharmacokinetic properties of the drug argue for therapeutic monitoring, but so far described analytical methods have shortcomings with respect to application in a routine setting. The aim of our work was to develop an analytical method suitable for routine use.

Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used. For sample preparation, protein precipitation followed by on-line solid phase extraction was used. SCH 56984, a posaconazole related compound provided by the manufacturer of posaconazole, was used as internal standard.

Results: The method was characterized by short hands-on time and an analytical run time of four minutes. Validation data demonstrated acceptable analytical performance.

Conclusions: LC-MS/MS with on-line solid phase extraction for sample preparation allows the implementation of a convenient and reliable method for pharmacokinetic monitoring of posaconazole.

Clin Chem Lab Med 2009;47:579–84.

Keywords: antimycotic drugs; fungal infections; liquid chromatography-tandem mass spectrometry (LC-MS/MS); posaconazole

About the article

Corresponding author: Michael Vogeser, MD, Institute of Clinical Chemistry, Hospital of the University of Munich, Marchioninistr. 15, 81377 Munich, Germany Phone: +49 89 7095 3221, Fax: +49 89 7095 6220,


Received: 2008-06-24

Accepted: 2009-02-17

Published Online: 2009-03-23

Published in Print: 2009-05-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.129.

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©2009 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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