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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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Volume 47, Issue 5


Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients

Eline Verhoye / Annick Van den Bruel / Joris R. Delanghe / Evi Debruyne / Michel R. Langlois


Background: Three adult patients presented with unexpectedly high thyrotropin (TSH) concentrations that were discordant with clinical and biochemical findings of euthyroid or hyperthyroid status.

Methods: Antibody interference in the TSH immunoassay (Roche) was investigated by polyethylene glycol (PEG)-pretreatment, heterophilic blocking tube (HBT)-pretreatment, rheumatoid factor (RF) testing, immunofixation, protein A adsorption, and gel filtration chromatography (GFC).

Results: PEG-precipitation yielded <20% recovery of serum TSH, whereas HBT-pretreatment did not decrease TSH test results. RF-testing and immunofixation were negative. Protein A adsorption and GFC demonstrated the presence of TSH-immunoglobulin complexes in serum.

Conclusions: Interference by TSH-immunoglobulin complexes should be ruled out in euthyroid and hyperthyroid patients presenting with inappropriately increased or non-suppressed TSH values.

Clin Chem Lab Med 2009;47:604–6.

Keywords: antibody interference; electrochemiluminescent immunoassay (ECLIA); polyethylene glycol (PEG); thyroid-stimulating hormone (TSH)

About the article

Corresponding author: Michel R. Langlois, Department of Clinical Chemistry, AZ St-Jan, Ruddershove 10, 8000 Bruges, Belgium Phone: +32-50-452729, Fax: +32-50-452619,

Received: 2009-01-06

Accepted: 2009-03-10

Published in Print: 2009-05-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 5, Pages 604–606, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.138.

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