Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 47, Issue 6


Proteomics of exhaled breath: methodological nuances and pitfalls

Viktoria S. Kurova / Eldar C. Anaev / Alexey S. Kononikhin / Kristina Yu. Fedorchenko / Igor A. Popov / Timothey L. Kalupov / Dmitriy O. Bratanov / Eugenie N. Nikolaev / Sergey D. Varfolomeev


Background: The analysis of exhaled breath condensate (EBC) can be an alternative to traditional endoscopic sampling of lower respiratory tract secretions. This is a simple non-invasive method of diagnosing respiratory diseases, in particular, respiratory inflammatory processes.

Methods: Samples were collected with a special device-condenser (ECoScreen, VIASYS Healthcare, Germany), then treated with trypsin according to the proteomics protocol for standard protein mixtures and analyzed by nanoflow high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) with a 7-Tesla Finnigan LTQ-FT mass spectrometer (Thermo Electron, Germany). Mascot software (Matrixscience) was used for screening the database NCBInr for proteins corresponding to the peptide maps that were obtained.

Results: EBCs from 17 young healthy non-smoking donors were collected. Different methods for concentrating protein were compared in order to optimize EBC preparations for proteomic analysis. The procedure that was chosen allowed identification of proteins exhaled by healthy people. The major proteins in the condensates were cytoskeletal keratins. Another 12 proteins were identified in EBC from healthy non-smokers. Some keratins were found in the ambient air and may be considered exogenous components of exhaled air.

Conclusions: Knowledge of the normal proteome of exhaled breath allows one to look for biomarkers of different disease states in EBC. Proteins in ambient air can be identified in the respiratory tract and should be excluded from the analysis of the proteome of EBC. The results obtained allowed us to choose the most effective procedure of sample preparation when working with samples containing very low protein concentrations.

Clin Chem Lab Med 2009;47:706–12.

Keywords: exhaled breath condensate (EBC); nanoflow HPLC-tandem mass spectrometry; proteomics

About the article

Corresponding author: Dr. Viktoria Kurova, N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4/11 Kosygin Street, 119334 Moscow, Russian Federation Fax: +7 495 137 41 01,

Received: 2008-11-26

Accepted: 2009-04-21

Published in Print: 2009-06-01

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 6, Pages 706–712, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.166.

Export Citation

©2009 by Walter de Gruyter Berlin New York.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Lan Huang, Dan Shao, Yan Wang, Xueteng Cui, Yufei Li, Qian Chen, and Juan Cui
Briefings in Bioinformatics, 2020
Olof Beck, Anna-Carin Olin, and Ekaterina Mirgorodskaya
Clinical Chemistry, 2016, Volume 62, Number 1, Page 84
Alexey S. Kononikhin, Alexander G. Brzhozovskiy, Anna M. Ryabokon, Kristina Fedorchenko, Natalia V. Zhakharova, Alexander I. Spasskii, Igor A. Popov, Vyacheslav K. Ilyin, Zoya O. Solovyova, Lyudmila Kh. Pastushkova, Alexey V. Polyakov, Sergey D. Varfolomeev, Irina M. Larina, and Evgeny N. Nikolaev
International Journal of Molecular Sciences, 2019, Volume 20, Number 18, Page 4518
Daniela Pelclova, Pavel Talacko, Tomas Navratil, Blanka Zamostna, Zdenka Fenclova, Stepanka Vlckova, and Sergey Zakharov
Monatshefte für Chemie - Chemical Monthly, 2019, Volume 150, Number 9, Page 1715
Laura Núñez-Naveira, Luis Antonio Mariñas-Pardo, and Carmen Montero-Martínez
Lung, 2019, Volume 197, Number 4, Page 523
Ildiko Horváth, Peter J. Barnes, Stelios Loukides, Peter J. Sterk, Marieann Högman, Anna-Carin Olin, Anton Amann, Balazs Antus, Eugenio Baraldi, Andras Bikov, Agnes W. Boots, Lieuwe D. Bos, Paul Brinkman, Caterina Bucca, Giovanna E. Carpagnano, Massimo Corradi, Simona Cristescu, Johan C. de Jongste, Anh-Tuan Dinh-Xuan, Edward Dompeling, Niki Fens, Stephen Fowler, Jens M. Hohlfeld, Olaf Holz, Quirijn Jöbsis, Kim Van De Kant, Hugo H. Knobel, Konstantinos Kostikas, Lauri Lehtimäki, Jon O. Lundberg, Paolo Montuschi, Alain Van Muylem, Giorgio Pennazza, Petra Reinhold, Fabio L.M. Ricciardolo, Philippe Rosias, Marco Santonico, Marc P. van der Schee, Frederik-Jan van Schooten, Antonio Spanevello, Thomy Tonia, and Teunis J. Vink
European Respiratory Journal, 2017, Volume 49, Number 4, Page 1600965
V. S. Kurova, E. C. Anaev, A. S. Kononikhin, I. A. Popov, K. Yu. Fedorchenko, E. N. Nikolaev, S. D. Varfolomeev, and A. G. Chuchalin
Russian Chemical Bulletin, 2010, Volume 59, Number 1, Page 292
J. Brennan McNeil, Ciara M. Shaver, V. Eric Kerchberger, Derek W. Russell, Brandon S. Grove, Melissa A. Warren, Nancy E. Wickersham, Lorraine B. Ware, W. Hayes McDonald, and Julie A. Bastarache
American Journal of Respiratory and Critical Care Medicine, 2018, Volume 197, Number 8, Page 1027
Laura M. López-Sánchez, Bernabé Jurado-Gámez, Nuria Feu-Collado, Araceli Valverde, Amanda Cañas, José L. Fernández-Rueda, Enrique Aranda, and Antonio Rodríguez-Ariza
American Journal of Physiology-Lung Cellular and Molecular Physiology, 2017, Volume 313, Number 4, Page L664
Vladimir G. Zaikin and Alexander A. Sysoev
European Journal of Mass Spectrometry, 2013, Volume 19, Number 6, Page 399
K. Yu. Fedorchenko, A. M. Ryabokon’, A. S. Kononikhin, S. I. Mitrofanov, E. A. Mikhant’eva, A. I. Spasskii, I. R. Sukhodolov, I. A. Popov, A. V. Polyakov, I. M. Larina, E. N. Nikolaev, and S. D. Varfolomeev
Russian Chemical Bulletin, 2016, Volume 65, Number 11, Page 2745
Raul Emrich Melo, Todor A Popov, and Dirceu Solé
Jornal Brasileiro de Pneumologia, 2010, Volume 36, Number 6, Page 693
A.S. Kononikhin, N.L. Starodubtseva, V.V. Chagovets, A.Y. Ryndin, A.A. Burov, I.A. Popov, A.E. Bugrova, R.A. Dautov, A.O. Tokareva, Y.L. Podurovskaya, O.V. Ionov, V.E. Frankevich, E.N. Nikolaev, and G.T. Sukhikh
Journal of Chromatography B, 2017, Volume 1047, Page 97
Cristina Airoldi, Carlotta Ciaramelli, Marco Fumagalli, Rita Bussei, Valeria Mazzoni, Simona Viglio, Paolo Iadarola, and Jan Stolk
Journal of Proteome Research, 2016, Volume 15, Number 12, Page 4569
A. S. Kononikhin, K. Yu. Fedorchenko, A. M. Ryabokon, N. L. Starodubtseva, I. A. Popov, M. G. Zavialova, E. C. Anaev, A. G. Chuchalin, S. D. Varfolomeev, and E. N. Nikolaev
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2016, Volume 10, Number 3, Page 230
S. D. Varfolomeev, S. V. Lushchekina, and A. V. Nemukhin
Herald of the Russian Academy of Sciences, 2016, Volume 86, Number 3, Page 185
Sarah A Hayes, Simon Haefliger, Benjamin Harris, Nick Pavlakis, Stephen J Clarke, Mark P Molloy, and Viive M Howell
Journal of Breath Research, 2016, Volume 10, Number 3, Page 034001
A. S. Kononikhin, V. V. Chagovets, N. L. Starodubtseva, A. Y. Ryndin, A. E. Bugrova, Y. I. Kostyukevich, I. A. Popov, V. E. Frankevich, O. V. Ionov, G. T. Sukhikh, and E. N. Nikolaev
Molecular Biology, 2016, Volume 50, Number 3, Page 470
K. U. Fedorchenko, A. M. Ryabokon, A. S. Kononikhin, S. I. Mitrofanov, V. V. Barmin, O. V. Pikin, E. H. Anaev, I. V. Gachok, I. A. Popov, E. N. Nikolaev, A. G. Chuchalin, and S. D. Varfolomeev
Moscow University Chemistry Bulletin, 2016, Volume 71, Number 2, Page 134
A. S. Kononikhin, A. Yu. Ryndin, N. L. Starodubtseva, V. V. Chagovets, A. A. Burov, A. E. Bugrova, Yu. I. Kostyukevich, I. A. Popov, V. E. Frankevich, O. V. Ionov, V. V. Zubkov, and E. N. Nikolaev
Bulletin of Experimental Biology and Medicine, 2016, Volume 160, Number 6, Page 861
Joseph E. Levitt and Angela J. Rogers
Expert Review of Proteomics, 2016, Volume 13, Number 5, Page 457
Sean Harshman, Claude Grigsby, and Darrin Ott
Chromatography, 2014, Volume 1, Number 3, Page 108
A.S. Kononikhin, K.Yu. Fedorchenko, A.M. Ryabokon, N.L. Starodubtseva, I.A. Popov, M.G. Zavialova, E.C. Anaev, A.G. Chuchalin, S.D. Varfolomeev, and E.N. Nikolaev
Biomeditsinskaya Khimiya, 2015, Volume 61, Number 6, Page 777
S. R. Corrie, J. W. Coffey, J. Islam, K. A. Markey, and M. A. F. Kendall
The Analyst, 2015, Volume 140, Number 13, Page 4350
Vera Muccilli, Rosaria Saletti, Vincenzo Cunsolo, Jenny Ho, Elisa Gili, Enrico Conte, Stefania Sichili, Carlo Vancheri, and Salvatore Foti
Journal of Pharmaceutical and Biomedical Analysis, 2015, Volume 105, Page 134
Marco Fumagalli, Fabio Ferrari, Maurizio Luisetti, Jan Stolk, Pieter Hiemstra, Daniela Capuano, Simona Viglio, Laura Fregonese, Isa Cerveri, Federica Corana, Carmine Tinelli, and Paolo Iadarola
International Journal of Molecular Sciences, 2012, Volume 13, Number 12, Page 13894
Simona Viglio, Jan Stolk, Paolo Iadarola, Serena Giuliano, Maurizio Luisetti, Roberta Salvini, Marco Fumagalli, and Anna Bardoni
Proteomes, 2014, Volume 2, Number 1, Page 18
Philippe Rosias
Journal of Breath Research, 2012, Volume 6, Number 2, Page 027102
V.S. Kurova, I.N. Kurochkin, G.R. Kalamkarov, A.E. Bugrova, K.Yu. Fedortchenko, and S.D. Varfolomeev
Biotechnology Advances, 2009, Volume 27, Number 6, Page 945
Begoña Casado, Maurizio Luisetti, and Paolo Iadarola
Expert Review of Clinical Immunology, 2011, Volume 7, Number 1, Page 111
V. S. Kurova, A. S. Kononikhin, D. A. Sakharov, I. A. Popov, I. M. Larina, A. G. Tonevitsky, S. D. Varfolomeev, and E. N. Nikolaev
Russian Journal of Bioorganic Chemistry, 2011, Volume 37, Number 1, Page 48

Comments (0)

Please log in or register to comment.
Log in