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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 47, Issue 7

Issues

Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting

Vasco Lança
  • Division of Endocrinology, Diabetes and Hypertension, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA
  • Vasco Lança and Robert Y.L. Zee contributed equally to the study.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Robert Y.L. Zee
  • Division of Preventive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA
  • Vasco Lança and Robert Y.L. Zee contributed equally to the study.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alicia Rivera
  • Departments of Laboratory Medicine and Pathology, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jose R. Romero
  • Division of Endocrinology, Diabetes and Hypertension, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2009-06-04 | DOI: https://doi.org/10.1515/CCLM.2009.188

Abstract

Background: There is accumulating evidence from the epidemiological field of telomere biology that telomere length plays an important role in the pathophysiology of cardiovascular disease. The RNA-dependent DNA polymerase, telomerase, is essential in regulating telomere length by acting as a reverse transcriptase. However, the relationship between telomerase activity and telomere length in cardiovascular disease is unclear. This is due, in part, to the paucity of information on the utility of a quantitative and routine assay for the determination of telomerase activity in circulating blood leukocytes.

Methods: We used a validated, high-sensitive real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) to determine telomerase activity in circulating blood leukocytes.

Results: The present investigation demonstrated direct and reliable detection of telomerase activity of circulating blood leukocytes.

Conclusion: The present investigation suggests the feasibility of using RQ-TRAP assay in routine screening of telomerase activity in blood specimens typically collected in a clinical/epidemiological setting.

Clin Chem Lab Med 2009;47:870–3.

Keywords: circulating leukocytes; real-time quantitative telomeric repeat amplification protocol (RQ-TRAP); telomerase activity

About the article

Corresponding authors: Dr. Robert Y.L. Zee, Division of Preventive Medicine, Brigham and Women's Hospital and Harvard Medical School, 900 Commonwealth Avenue East, Boston, MA 02215, USA Dr. Jose R. Romero, Division of Endocrinology, Diabetes and Hypertension, Brigham and Women's Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA


Received: 2009-02-06

Accepted: 2009-04-06

Published Online: 2009-06-04

Published in Print: 2009-07-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 47, Issue 7, Pages 870–873, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.188.

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