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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

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Online
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1437-4331
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Volume 47, Issue 9 (Sep 2009)

Issues

Preeclamptic placental stress and over expression of mitochondrial HSP70

Ekambaram Padmini
  • P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai, Tamilnadu, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Srinivasan Lavanya
  • P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai, Tamilnadu, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Venkatraman Uthra
  • P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai, Tamilnadu, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Background: Evidence is accumulating that mitochondrial (Mt) oxidative stress plays a role in the pathogenesis of preeclampsia. The current study analyzes the stress levels, energy status and associated enzymatic alteration in placental mitochondria of preeclamptic (n=30) and normotensive (n=35) subjects.

Methods: Total Mt stress was measured using dichlorofluorescin (DCFH) oxidant analysis, malondialdehyde (MDA) concentrations, protein carbonyl (PC) concentrations and measurement of nitrite (NO2) and nitrate (NO3). Activity of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx) and the glutathione redox ratio (GRR) were measured. The ATP/ADP (adenosine triphosphate/adenosine diphosphate) concentrations and respiratory chain enzyme activities were also analyzed. The expression of heat shock protein 70 (HSP70) was measured in mitochondria.

Results: The DCFH oxidants, MDA, PC concentrations, and concentrations of NO2 and NO3 were significantly higher in the preeclamptic group (p<0.01) compared with the control group. The activities of SOD, GPx, GRR [glutathione (GSH)/glutathione disulfate (GSSG)] (p<0.01, p<0.001), ATP/ADP and respiratory chain enzyme activities were reduced significantly (p<0.001) in preeclamptic conditions. The placental mitochondrial HSP70 (mtHSP70) showed significant over expression in the preeclamptic group (p<0.001) compared with the control group.

Conclusions: These results provide the first line of evidence for accumulated Mt stress demonstrated by increased stress markers, decreased antioxidants and enhanced mtHSP70. The study illustrates the probable protective mechanism of mtHSP70 against the generated stress. This is primarily to combat the enzymatic and free radical mediated damage produced in preeclampsia.

Clin Chem Lab Med 2009;47:1073–80.

Keywords: antioxidants; energy status; heat shock protein 70 (HSP70); oxidative stress; preeclampsia

About the article

Corresponding author: Dr. E. Padmini, Reader in Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai – 600108, Tamilnadu, India Phone: +91-044-26213748, Fax: +91-044-25280473, ;


Received: 2009-03-25

Accepted: 2009-06-16

Published in Print: 2009-09-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2009.247.

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©2009 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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