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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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IMPACT FACTOR 2016: 3.432

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1437-4331
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In This Section
Volume 48, Issue 1 (Jan 2010)

Issues

Analytical performance of the serum free light chain assay

Pierre-Yves Briand
  • Laboratoire de Biochimie Générale et Enzymologie, CHU Pontchaillou, Rennes, France
/ Olivier Decaux
  • Service de Médecine Interne, CHU Hôpital Sud, Rennes, France
/ Hélène Caillon
  • Laboratoire de Biochimie Générale et Enzymologie, CHU Pontchaillou, Rennes, France
/ Bernard Grosbois
  • Service de Médecine Interne, CHU Hôpital Sud, Rennes, France
/ André Le Treut
  • Laboratoire de Biochimie Générale et Enzymologie, CHU Pontchaillou, Rennes, France
/ Lucienne Guenet
  • Laboratoire de Biochimie Générale et Enzymologie, CHU Pontchaillou, Rennes, France
Published Online: 2009-11-25 | DOI: https://doi.org/10.1515/CCLM.2010.012

Abstract

Background: The Freelite™ system for nephelometric or turbidimetric measurement of serum free light chains (FLCs) has been available since 2001. It has been valuable for the management of patients with oligosecretory myeloma, light chain myeloma and AL amyloidosis. However, there are several limitations of the method. The goal of this study was to evaluate the analytical performance of the FLC assay.

Methods: Titrated controls and clinical serum specimens were used to determine precision and post-dilution recovery.

Results: As reported elsewhere, we found that the assay had several limitations, including poor post-dilution linearity and overestimation by nephelometry.

Conclusions: These data demonstrate that the results of the FLC assay must be interpreted jointly by the clinician and the biologist, taking into account the individual patient's clinical and biological characteristics.

Clin Chem Lab Med 2010;48:73–9.

Keywords: free light chains; immunoassay; monoclonal gammopathy

About the article

Corresponding author: Pierre-Yves Briand, Laboratoire de Biochimie Générale et Enzymologie, 2 rue Henri Le Guilloux, 35033 Rennes Cedex 9, France


Received: 2009-04-04

Accepted: 2009-09-08

Published Online: 2009-11-25

Published in Print: 2010-01-01



Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.012. Export Citation

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