Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 48, Issue 1 (Jan 2010)

Issues

A study on the stability of urinary free catecholamines and free methyl-derivatives at different pH, temperature and time of storage

Norman B. Roberts
  • Department of Clinical Biochemistry, The Royal Liverpool and Broadgreen University Hospitals, Liverpool, UK
/ Gerald Higgins
  • Department of Clinical Biochemistry, The Royal Liverpool and Broadgreen University Hospitals, Liverpool, UK
/ Mansour Sargazi
  • Department of Clinical Biochemistry, The Royal Liverpool and Broadgreen University Hospitals, Liverpool, UK
Published Online: 2009-11-25 | DOI: https://doi.org/10.1515/CCLM.2010.017

Abstract

Bakground: The goal of our study was to test the relative stability of urine, unconjugated, free catecholamines and the methyl derivatives. We measured the change in concentrations in commercially available urines after storage at various pH values, temperatures and time, from days up to 10 weeks.

Methods: Samples of commercial control urines were adjusted to pH 2.0, 4.0, 6.0 and 8.0 and aliquots stored at ambient temperature (20–26°C), 4°C and –18oC. The free catecholamines (cats) and the free methyl derivatives (mets) were measured after 1, 2, 3 and 6 days and 1, 2, 3 and 10 weeks using the automated sample trace enrichment dialysis (ASTED) procedure with reversed phase ion pair high performance liquid chromatography (HPLC) and coulometric detection.

Results: Free catecholamines were relatively stable, with <15% loss of concentration, when stored at pH 6.0 or less for at least 4 days and up to 10 weeks at pH 2.0 at either 4oC or –18oC. At pH 8.0, the concentration fell to <60% after 48 h and at a pH of 6.0 or 8.0, up to 90% was lost within the first week at 4oC and 25oC. More than 40% of free normetadrenaline and metadrenaline were lost after 1–2 weeks when stored at 20–25oC and pH 8.0. After 10 weeks at pH 4.0, 6.0 and 8.0, up to 90% loss was observed at 25oC. Free cats were stable at pH 2.0 and 4.0 at –18oC and the free mets were stable at –18oC over the entire time period studied and at all pHs.

Conclusions: In the analysis of free catecholamine and the free methyl derivatives, urine samples should be acidified to a pH range 2.0–3.0 to ensure stability and hence the correct analysis.

Clin Chem Lab Med 2010;48:81–7.

Keywords: free catecholamines; methylated derivatives; stability; urine

About the article

Corresponding author: Norman B. Roberts, The Department of Clinical Biochemistry, The Royal Liverpool and Broadgreen University Hospitals, Liverpool, UK


Received: 2009-06-18

Accepted: 2009-09-20

Published Online: 2009-11-25

Published in Print: 2010-01-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.017.

Export Citation

©2010 by Walter de Gruyter Berlin New York. Copyright Clearance Center

Comments (0)

Please log in or register to comment.
Log in