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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

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Source Normalized Impact per Paper (SNIP) 2017: 1.188

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1437-4331
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Volume 48, Issue 11

Issues

Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia

Yu-Jie Gao
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Yu-Juan He
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Zai-Lin Yang
  • Center for Hematology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China
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/ Hui-Yuan Shao
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Yan Zuo
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Yao Bai
  • Department of Laboratory Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China
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/ Hui Chen
  • Department of Laboratory Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China
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/ Xian-Chun Chen
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Feng-Xian Qin
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Shi Tan
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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/ Jian Wang
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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  • De Gruyter OnlineGoogle Scholar
/ Li Wang
  • Department of Hematology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China
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/ Ling Zhang
  • Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing, P.R. China
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Published Online: 2010-09-13 | DOI: https://doi.org/10.1515/CCLM.2010.311

Abstract

Background: Increased cell-free DNA (cf-DNA) and the integrity of cf-DNA in plasma of patients with cancer has been described. We investigated the clinical utility of cf-DNA in the detection and monitoring of progression of leukemia.

Methods: Plasma samples from 60 patients with acute leukemia were analyzed in comparison to plasma from 30 healthy controls. Plasma DNA was determined by quantitative PCR (qPCR) by amplifying the β-actin gene (ACTB). The DNA integrity index was calculated as the ratio of qPCR results (ACTB384/106). Paired diagnostic/complete remission (CR)/relapse samples from eight of 60 patients were analyzed, and the minimum residual disease (MRD) situations were monitored.

Results: DNA concentrations (median: 8.80 ng/mL, p=0.004) and DNA integrity (median: 0.51, p<0.001) in cancer patients were significantly higher. Receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve of DNA and DNA integrity were 0.79 and 0.88, respectively. DNA integrity at CR had a distinct reduction and then an increase at relapse. DNA integrity in CR cases was higher than that observed in healthy controls.

Conclusions: Our preliminary data suggest that plasma DNA integrity is increased in acute leukemia and may be a potential biomarker for monitoring MRD. However, more work is needed.

Clin Chem Lab Med 2010;48:1651–6.

Keywords: acute leukemia; cell-free DNA; DNA; integrity; plasma

About the article

Corresponding author: Ling Zhang, Faculty of Laboratory Medicine, Chongqing Medical University, 1#, Yixueyuan Road, 400016, Chongqing, P.R. China Phone: +86 023-68485223, Fax: +86 023-68485005,


Received: 2009-12-21

Accepted: 2010-05-12

Published Online: 2010-09-13

Published in Print: 2010-11-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 48, Issue 11, Pages 1651–1656, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.311.

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