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Clinical Chemistry and Laboratory Medicine (CCLM)

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First certified reference material for cystatin C in human serum ERM-DA471/IFCC

Anders Grubb1 / Søren Blirup-Jensen1 / Veronica Lindström1 / Camilla Schmidt1 / Harald Althaus2 / Ingrid Zegers3 /

1Department of Clinical Chemistry, University Hospital, Lund, Sweden

2Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany

3Institute for Reference Materials and Measurements, Joint Research Centre, European Commission, Geel, Belgium

Corresponding author: Ingrid Zegers, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, Geel 2440, Belgium Phone: +32-14-571920, Fax: +32-14-571548,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 48, Issue 11, Pages 1619–1621, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.318, October 2010

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Published Online:


The IFCC Working Group for the Standardisation of Cystatin C (WG-SCC), in collaboration with the Institute for Reference Materials and Measurements (IRMM), announces the availability of the new certified reference material ERM-DA471/IFCC. The material was characterised using a pure protein primary reference preparation (PRP) as calibrant. The PRP was prepared from recombinant cystatin C, and its concentration measured using dry mass determination. The characterisation of ERM-DA471/IFCC was performed by particle enhanced immuno-nephelometry, particle enhanced immuno-turbidimetry, and enzyme amplified single radial immuno-diffusion. The certified cystatin C mass concentration in ERM-DA471/IFCC, if reconstituted according to the specified procedure, is 5.48 mg/L, the expanded uncertainty (k=2) being 0.15 mg/L.

Clin Chem Lab Med 2010;48:1619–21.

Keywords: certified reference material; cystatin C; glomerular filtration rate; immunoassay

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