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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
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Volume 48, Issue 12 (Dec 2010)

Issues

Development and validation of a combined method for the biomonitoring of omega-3/-6 fatty acids and conjugated linoleic acids in different matrices from human and nutritional sources

Christian Böcking
  • Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, Germany
/ Wolfgang Andreas Nockher
  • Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, Germany
/ Matthias Schreiner
  • Department of Food Science and Technology, BOKU-University of Natural Resources and Applied Life Sciences Vienna, Austria
/ Harald Renz
  • Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, Germany
/ Petra Ina Pfefferle
  • Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, Germany
Published Online: 2010-10-25 | DOI: https://doi.org/10.1515/CCLM.2010.350

Abstract

Background: During the last decade, the contribution of omega-3 and -6 long chain-polyunsaturated fatty acids (LC-PUFA) and conjugated linoleic acids (CLA) to the prevention and development of many inflammatory and cardiovascular diseases has been of growing interest. In order to investigate the etiology of these diseases, rapid, combined and comparable methods are invaluable for monitoring both the intake and the incorporation of these fatty acids (FA).

Methods: The fatty acid methyl esters (FAME) were analyzed using a gas chromatography/flame ionization detector (GC-FID) system and quantified with an internal standard (C18:0 iso).

Results: An effective and rapid protocol for sample preparation and the analysis of FAME was developed and validated. The comparison of different extraction methods showed that the Hara and Radin method gave the best results for serum and erythrocyte membranes. Excellent mean within-day and day-to-day precisions for serum, erythrocytes and cow's milk LC-PUFAs demonstrated the high reproduci-bility of the method. Recovery rates for FAMEs in serum and milk were close to 100%. In addition, high mean method linearity (R2) (>0.99) was shown for serum, erythrocytes and cow's milk. The sensitivity for FA achieved by GC analysis was acceptable.

Conclusion: With the newly adapted protocols, combined and rapid analyses of up to 46 FAMEs, including CLAs and omega-3/-6 LC-PUFAs, can be conducted with high reliability and reproducibility using serum, erythrocyte membranes or cow's milk. This provides a novel tool that can be easily implemented in epidemiological studies or clinical diagnostics.

Clin Chem Lab Med 2010;48:1757–63.

Keywords: conjugated linoleic acid; erythrocytes; gas chromatography; milk; PUFA analysis; serum

About the article

Corresponding author: Petra Ina Pfefferle, Department of Clinical Chemistry and Molecular Diagnostics, Philipps-University of Marburg, Biomedical Research Centre, Hans-Meerwein-Str 2, 35043 Marburg, Germany


Received: 2010-03-22

Accepted: 2010-05-16

Published Online: 2010-10-25

Published in Print: 2010-12-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.350.

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©2010 by Walter de Gruyter Berlin New York. Copyright Clearance Center

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