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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 48, Issue 4

Issues

A methodological reappraisal of total and high molecular weight adiponectin determination in human peripheral circulation: comparison of four immunometric assays

Chiara Caselli
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ombretta Melaiu
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maristella Maltinti / Silvia Del Ry
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Fondazione Toscana Gabriele Monasterio, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Manuela Cabiati / Tommaso Prescimone
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Danilo Neglia
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Fondazione Toscana Gabriele Monasterio, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Daniela Giannessi
  • Laboratory of Cardiovascular Biochemistry, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
  • Fondazione Toscana Gabriele Monasterio, Pisa, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Background: Adiponectin, present in different multimeric forms in circulation, is increasingly used in clinical settings as a cardiometabolic marker. The development of several commercially available enzyme-linked immunosorbent assays (ELISA) has allowed for the widespread measurement of adiponectin in research as well as in clinical practice. The comparative performance of these assays is thus an issue of major relevance.

Methods: The analytical performance of four different ELISAs (LINCO, B-Bridge, SPIbio and ALPCO) was evaluated. Samples from 102 cardiac patients and 40 healthy subjects were tested using LINCO and ALPCO. The latter is able to measure the concentrations of multimers. For the multimer assay, an error propagation study was performed. A subset of subjects was tested by all four ELISAs for comparison purposes.

Results: Bland-Altman plots revealed differences between the results of the four ELISAs, mainly for ALPCO. However, a strong correlation between the different ELISAs (ALPCO, r=0.83; B-Bridge, r=0.98; SPIbio, r=0.91 vs. LINCO) was observed. Total adiponectin measured by LINCO showed a better association with cardiac disease [receiver-operating characteristic (ROC) curves] than total and high molecular weight adiponectin measured by ALPCO.

Conclusions: The results of this study contribute to a better understanding of the methodological features of the several ELISAs, and help in the evaluation and comparison of the relative results.

Clin Chem Lab Med 2010;48:561–8.

Keywords: adiponectin; adiponectin multimers; cardiovascular disease; ELISA; high molecular weight

About the article

Corresponding author: Dr Daniela Giannessi, Laboratory of Cardiovascular Biochemistry, CNR Institute of Clinical Physiology, Pisa Research Area, Via G. Moruzzi, 1 56100 Pisa, Italy Phone: +39 050 3152664, Fax: +39 050 3152166, .


Received: 2009-09-02

Accepted: 2009-12-02

Published in Print: 2010-04-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 48, Issue 4, Pages 561–568, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2010.104.

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